Key Laboratory of Forest Plant Ecology, Ministry of Education, Northeast Forestry University, 150040 Harbin, PR China; Engineering Research Center of Forest Bio-preparation, Ministry of Education, Northeast Forestry University, Harbin 150040, China; The College of Chemistry, Chemical Engineering and Resource Utilization, Northeast Forestry University, 150040 Harbin, PR China.
The College of Forestry, Beijing Forestry University, 100083 Beijing, PR China.
Int J Biol Macromol. 2023 Dec 31;253(Pt 6):126794. doi: 10.1016/j.ijbiomac.2023.126794. Epub 2023 Sep 11.
Targeted separation of active phytochemicals is urgently needed in the natural medicine field. In this paper, due to the natural porosity and high biocompatibility of cellulose, a nanocellulose membrane combined with surface molecular imprinting was successfully prepared; the efficient nanocellulose-based molecular imprinted membrane (NC-MIM) provided good adsorption for the targeted separation of phytochemicals such as 10-deacetylbaccatin III (10-DAB), an essential intermediate in the synthesis of the anticancer drug paclitaxel. Through a series of characterization and adsorption experiments, the adsorption mechanism of NC-MIM was determined. At pH 8.0 and temperatures of 20 °C-40 °C, the maximum capacity of NC-MIM for adsorption of 10-DAB reached 66.90 mg g , and the content of 10-DAB was dramatically increased 17.5-fold after adsorption. The specific adsorption results showed that NC-MIM had excellent capacity for targeted separation of 10-DAB from among taxane structural analogues. Even after ten cycles, NC-MIM demonstrated a remarkable adsorption capacity of 86.43%, thereby indicating exceptional selectivity and stability. The successful implementation of NC-MIM for green, safe, and efficient enrichment of phytochemicals from plants provides a promising new approach and valuable insights into its practical application.
靶向分离天然药物中的活性植物化学物质是目前亟待解决的问题。本文利用纤维素的天然多孔性和良好的生物相容性，成功制备了一种纳米纤维素膜，并结合表面分子印迹技术；高效的基于纳米纤维素的分子印迹膜（NC-MIM）为靶向分离 10-去乙酰巴卡丁 III（10-DAB）等植物化学物质提供了良好的吸附性能，10-DAB 是合成抗癌药物紫杉醇的重要中间体。通过一系列的表征和吸附实验，确定了 NC-MIM 的吸附机制。在 pH 8.0 和 20-40°C 的温度下，NC-MIM 对 10-DAB 的最大吸附容量达到 66.90mg/g，吸附后 10-DAB 的含量显著增加了 17.5 倍。特异性吸附结果表明，NC-MIM 对紫杉烷结构类似物中 10-DAB 的靶向分离具有优异的性能。即使经过十次循环，NC-MIM 仍表现出出色的吸附能力，达到 86.43%，表明其具有出色的选择性和稳定性。NC-MIM 成功用于从植物中绿色、安全、高效地富集植物化学物质，为其实际应用提供了一种有前途的新方法和有价值的见解。
