School of Materials and Environmental Engineering, Shenzhen Polytechnic, Shenzhen 518055, China.
Food Inspection & Quarantine Center, Shenzhen Customs, Shenzhen 518045, China.
Molecules. 2022 Dec 30;28(1):310. doi: 10.3390/molecules28010310.
A novel malachite green molecularly imprinted membrane (MG-MIM) with specific selectivity for malachite green (MG) and leucomalachite green (LMG) was prepared using a hydrophobic glass fiber membrane as the polymer substrate, methyl violet as a template analog, 4-vinyl benzoic acid as the functional monomer, and ethyleneglycol dimethacrylate as the crosslinking agent. MG-MIM and non-imprinted membrane (NIM) were structurally characterized using scanning electron microscopy, surface area analyzer, Fourier-transform infrared spectrometer and synchronous thermal analyzer. The results showed that MG-MIM possessed a fluffier surface, porous and looser structure, and had good thermal stability. Adsorption properties of MG-MIM were investigated under optimal conditions, and adsorption equilibrium was reached in 20 min. The saturated adsorption capacities for MG and LMG were 24.25 ng·cm and 13.40 ng·cm, and the maximum imprinting factors were 2.41 and 3.20, respectively. Issues such as "template leakage" and "embedding" were resolved. The specific recognition ability for the targets was good and the adsorption capacity was stable even after five cycles. The proposed method was successfully applied for the detection of MG and LMG in real samples, and it showed good linear correlation in the range of 0 to 10.0 μg·L (R = 0.9991 and 0.9982), and high detection sensitivity (detection limits of MG and LMG of 0.005 μg/kg and 0.02 μg·kg in shrimp, and 0.005 μg/kg and 0.02 μg/kg in fish sample). The recoveries and relative standard deviations were in the range of 76.31-93.26% and 0.73-3.72%, respectively. The proposed method provides a simple, efficient and promising alternative for monitoring MG and LMG in aquatic products.
一种新型孔雀石绿分子印迹膜（MG-MIM），对孔雀石绿（MG）和隐色孔雀石绿（LMG）具有特异性选择性，以疏水性玻璃纤维膜为聚合物基底，以甲基紫为模板类似物，4-乙烯基苯甲酸为功能单体，乙二醇二甲基丙烯酸酯为交联剂。使用扫描电子显微镜、比表面积分析仪、傅里叶变换红外光谱仪和同步热分析仪对 MG-MIM 和非印迹膜（NIM）进行了结构表征。结果表明，MG-MIM 具有更蓬松的表面、多孔疏松的结构和良好的热稳定性。在最佳条件下研究了 MG-MIM 的吸附性能，20 分钟达到吸附平衡。MG 和 LMG 的饱和吸附容量分别为 24.25ng·cm 和 13.40ng·cm，最大印迹因子分别为 2.41 和 3.20。解决了“模板泄漏”和“嵌入”等问题。目标的特异性识别能力良好，即使经过五次循环，吸附容量也稳定。该方法成功应用于实际样品中 MG 和 LMG 的检测，在 0 至 10.0μg·L 范围内具有良好的线性相关性（MG 和 LMG 的 R 值分别为 0.9991 和 0.9982），检测灵敏度高（虾中 MG 和 LMG 的检测限为 0.005μg/kg 和 0.02μg·kg，鱼样中 MG 和 LMG 的检测限为 0.005μg/kg 和 0.02μg/kg）。回收率和相对标准偏差分别在 76.31-93.26%和 0.73-3.72%范围内。该方法为监测水产品中 MG 和 LMG 提供了一种简单、高效、有前途的替代方法。
