蒲公英提取物与全反式维 A 酸联合诱导人乳腺癌细胞毒性。

Combined dandelion extract and all-trans retinoic acid induces cytotoxicity in human breast cancer cells.

机构信息

Department of Clinical Biochemistry, School of Medicine, Ardabil University of Medical Sciences, Ardabil, Iran.

Department of Genetics and Pathology, School of Medicine, Ardabil University of Medical Sciences, Ardabil, Iran.

出版信息

Sci Rep. 2023 Sep 12;13(1):15074. doi: 10.1038/s41598-023-42177-z.

DOI:10.1038/s41598-023-42177-z

PMID:37700002

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10497591/

Abstract

Breast cancer is one of the most prevalent and deadly cancers among women worldwide. Recently, natural compounds have been widely used for the treatment of breast cancer. Present study evaluated antiproliferative and anti-metastasis activities of two natural compounds of dandelion and all-trans-retinoic acid (ATRA) in human MCF-7 and MDA-MB231 breast cancer cells. We also evaluated the expression of MMP-2, MMP-9, IL-1β, p53, NM23 and KAI1 genes. Data showed a clear additive cytotoxic effect in concentrations of 40 μM ATRA with 1.5 and 4 mg/ml of dandelion extract in MCF-7 and MDA-MB231 cells, respectively. In both cell lines, compared with the untreated cells, the expression levels of MMP-9 and IL-1β were significantly decreased while p53 and KAI1 expression levels were increased. Besides, MMP-2 and NM23 had different expressions in the two studied cell lines. In conclusion, dandelion/ATRA co-treatment, in addition to having strong cytotoxic effects, has putative effects on the expression of anti-metastatic genes in both breast cancer cells.

摘要

乳腺癌是全球女性中最常见和最致命的癌症之一。最近，天然化合物已被广泛用于治疗乳腺癌。本研究评估了蒲公英和全反式维甲酸（ATRA）两种天然化合物对人 MCF-7 和 MDA-MB231 乳腺癌细胞的增殖抑制和抗转移活性。我们还评估了 MMP-2、MMP-9、IL-1β、p53、NM23 和 KAI1 基因的表达。结果表明，在 MCF-7 和 MDA-MB231 细胞中，浓度为 40 μM 的 ATRA 与 1.5 和 4 mg/ml 的蒲公英提取物联合使用时，具有明显的相加细胞毒性作用。在这两种细胞系中，与未经处理的细胞相比，MMP-9 和 IL-1β 的表达水平显著降低，而 p53 和 KAI1 的表达水平增加。此外，MMP-2 和 NM23 在两种研究细胞系中的表达不同。总之，蒲公英/ATRA 联合治疗除了具有强烈的细胞毒性作用外，还可能对两种乳腺癌细胞中抗转移基因的表达产生影响。