Department of Medical Biology, Van Yüzüncü Yıl University, Van, Türkiye.
Department of Medical Biology, Van Yüzüncü Yıl University, Institute of Health Sciences, Van, Türkiye.
Drug Chem Toxicol. 2024 Sep;47(5):693-709. doi: 10.1080/01480545.2023.2257403. Epub 2023 Sep 13.
This study aimed to investigate the cytotoxic and apoptotic effects of and fungal extracts on HT-29 and HCT-116 colorectal cancer cell lines and to search the DNA damage and oxidative stress caused by these extracts. Accordingly, mushroom extracts were applied to colorectal cancer cell lines , and the IC result was obtained with the MTT test. According to the IC result, extract had the most effective cytotoxicity value among all used mushroom extracts. TAS, TOS, and NRF-2 tests were used to investigate the molecular effect of extract on oxidative stress; the DNA ladder test was performed to assess DNA damage, the Scratch assay method was applied for cell migration analysis, and the colony assay was used to determine the colony formation potential of the cells. The results showed that mushroom extract reduces cell proliferation, colony formation, and NRF-2, induces DNA damage, slows cell migration, and increases oxidative stress. This study shows that mushroom extract reduces cell proliferation through damaging cellular DNA and has a cytotoxic effect in colorectal cancer cell lines.
本研究旨在探讨 和 真菌提取物对 HT-29 和 HCT-116 结直肠癌细胞系的细胞毒性和凋亡作用，并寻找这些提取物引起的 DNA 损伤和氧化应激。因此，将蘑菇提取物应用于结直肠癌细胞系 ，并通过 MTT 试验获得 IC 结果。根据 IC 结果，在所有使用的蘑菇提取物中， 提取物具有最有效的细胞毒性值。TAS、TOS 和 NRF-2 试验用于研究 提取物对氧化应激的分子作用；DNA 梯试验用于评估 DNA 损伤，划痕试验方法用于细胞迁移分析，集落试验用于确定细胞的集落形成潜力。结果表明， 蘑菇提取物可降低细胞增殖、集落形成和 NRF-2，诱导 DNA 损伤，减缓细胞迁移，并增加氧化应激。本研究表明， 蘑菇提取物通过破坏细胞 DNA 来降低细胞增殖，并在结直肠癌细胞系中具有细胞毒性作用。
