Student Research Committee, Arak University of Medical Sciences, Arak, Iran.
Department of Immunology, School of Medicine, Arak University of Medical Sciences, Arak, Iran.
Biotech Histochem. 2023 Nov;98(5):353-359. doi: 10.1080/10520295.2023.2190620. Epub 2023 Mar 27.
Current conventional therapy for colorectal cancer includes surgery, radiation and chemotherapy, all of which produce side effects. Herbal medicine can control the side effects of conventional treatments. We investigated the synergistic effect of a mixture of Roscoe (Ginger) and extracts on colorectal cancer cell apoptosis in vitro. We prepared ethanolic extracts of ginger (GEE) and (GLEE). Cytotoxicity was evaluated using MTT assay and the half-maximal inhibitory concentration (IC) of each extract was calculated. The effect of these extracts on apoptosis in cancer cells was assessed using flow cytometry; Bax, Bcl2 and caspase-3 gene expression was evaluated using real-time PCR. GEE and GLEE decreased CT-26 cell viability significantly in a dose-dependent manner; however, the combined application of GEE + GLEE was most effective. Bax:Bcl-2 gene expression ratio, caspase-3 gene expression and the number of apoptotic cells were increased significantly in CT-26 cells treated at the IC level of each compound, especially in the GEE + GLEE treatment group. Combined ginger and extracts exhibited synergistic antiproliferative and apoptotic effects on colorectal cancer cells.
目前结直肠癌的常规治疗包括手术、放疗和化疗，这些方法都会产生副作用。草药可以控制常规治疗的副作用。我们研究了姜黄素和姜提取物混合物对体外结直肠癌细胞凋亡的协同作用。我们制备了姜（GEE）和姜黄（GLEE）的乙醇提取物。使用 MTT 测定法评估细胞毒性，并计算每种提取物的半最大抑制浓度（IC）。使用流式细胞术评估这些提取物对癌细胞凋亡的影响；使用实时 PCR 评估 Bax、Bcl2 和 caspase-3 基因表达。GEE 和 GLEE 以剂量依赖性方式显着降低 CT-26 细胞活力；然而，GEE+GLEE 的联合应用最为有效。在每种化合物的 IC 水平处理 CT-26 细胞时，Bax:Bcl-2 基因表达比、caspase-3 基因表达和凋亡细胞数量显着增加，尤其是在 GEE+GLEE 处理组中。姜黄素和姜提取物的联合应用对结直肠癌细胞表现出协同的增殖抑制和凋亡作用。
