Status of Biofilm Production and Vancomycin Resistance in Enterococcus in the Rural Population of Mathura, India.

Introduction Hospital-acquired or nosocomial infections caused by the rapidly emerging bacteria vancomycin-resistant enterococci can be dangerous and even fatal. Therefore, this study aimed to investigate the presence of enterococci in various clinical specimens along with their vancomycin resistance status and biofilm-producing capabilities. Methods A total of 164 species were isolated and further included in this study. Isolation and identification were done by the standard bacteriological procedure, antibiotic susceptibility testingwas done by clinical laboratory standard guidelines, and biofilm production test was done by microtiter plate methods. Results Among the total of 164 isolates, constituted 60.97% and constituted 39.02%. Maximum isolates were from urine samples. The prevalence of vancomycin-resistant was 6.70%, and 18.29% of isolates were biofilm producers. The sensitivity among the biofilm producers was maximum for linezolid (87.33%), followed by teicoplanin (86.43%) and vancomycin (79.64%). Conclusion High prevalence of enterococci was found in urine samples andbiofilm producers isolates were more antibiotic-resistant than non-biofilm producers.