Jain Sarika, Kumar Ashwani, Kashyap Bineeta, Kaur Iqbal R
Department of Microbiology, University College of Medical Sciences & Guru Teg Bahadur Hospital, Delhi, India.
Int J Appl Basic Med Res. 2011 Jul;1(2):80-3. doi: 10.4103/2229-516X.91149.
Emergence of high-level aminoglycoside and glycopeptide resistance has significantly contributed to the mortality, particularly in serious enterococcal infections.
This study was aimed to determine the prevalence of high-level gentamicin resistance (HLGR), high-level streptomycin resistance (HLSR) and vancomycin resistance in enterococcal isolates recovered from patients with bacteremia.
A total of 110 blood culture isolates of enterococci were recovered from septicemic patients. Routine antibiotic susceptibility testing was performed and screening for ampilcillin, high-level aminoglycoside resistance (HLAR) and high-level vancomycin resistance was done by agar screen method.
Out of 110 isolates, Enterococcus faecium accounted for 53% of these isolates, followed by Enterococcus fecalis (33%), Enterococcus casseliflavus (8%), Enterococcus raffinosus (4%) and Enterococcus dispar (2%). Resistance to ampicillin, HLGR, HLSR and HLAR was detected in 58%, 62%, 58% and 54% of the isolates, respectively. No isolate was resistant to vancomycin.
This study illustrates the high prevalence of HLAR in enterococci from patients with septicemia in our region, which emphasizes the need to predict synergy between beta-lactams and aminoglycosides for management of enterococcal infections.
高水平氨基糖苷类和糖肽类耐药的出现显著增加了死亡率，尤其是在严重的肠球菌感染中。
本研究旨在确定从菌血症患者中分离出的肠球菌分离株中高水平庆大霉素耐药（HLGR）、高水平链霉素耐药（HLSR）和万古霉素耐药的患病率。
从败血症患者中总共分离出110株肠球菌血培养分离株。进行常规抗生素敏感性试验，并通过琼脂筛选法对氨苄西林、高水平氨基糖苷类耐药（HLAR）和高水平万古霉素耐药进行筛查。
在110株分离株中，屎肠球菌占这些分离株的53%，其次是粪肠球菌（33%）、格氏肠球菌（8%）、棉子糖肠球菌（4%）和殊异肠球菌（2%）。分别在58%、62%、58%和54%的分离株中检测到对氨苄西林、HLGR、HLSR和HLAR的耐药性。没有分离株对万古霉素耐药。
本研究表明我们地区败血症患者的肠球菌中HLAR患病率很高，这强调了预测β-内酰胺类和氨基糖苷类之间协同作用以管理肠球菌感染的必要性。
