Liu Yao, Chen Xinghua, Luifu Junwei, Zhao Jing, He Xujun, Xie Tengfei
Guangdong Eco-engineering Polytechnic Guangzhou China.
Food Sci Nutr. 2023 Jun 27;11(9):5565-5572. doi: 10.1002/fsn3.3513. eCollection 2023 Sep.
Ready-to-eat (RTE) foods are widely marketed in China and are important components of everyday diet. In this study, a total of 2000 RTE food samples were analyzed, 252 (12.60%) of which were positive for Enterobacteriaceae, and 48 were identified as containing extended-spectrum β-lactamase (ESBL)-producing isolates. Furthermore, the antimicrobial resistance patterns of these isolates to 14 antimicrobial agents revealed that most isolates were resistant to aminoglycosides and β-lactam antibiotics. The TEM-type gene was prevalent in our isolates (79.17%). The isolates ( = 48) were classified into three clusters based on the ERIC-PCR results. Forty-eight sequence types were found without duplicates, revealing genetic variation and relatedness among isolates. Thus, the results demonstrated the presence of ESBL-producing in Chinese RTE foods. The results of this study provide insights into the spread of antibiotic-resistant strains and improve understanding of microbial risks.
即食（RTE）食品在中国广泛销售，是日常饮食的重要组成部分。在本研究中，共分析了2000份即食食品样本，其中252份（12.60%）肠杆菌科呈阳性，48份被鉴定为含有产超广谱β-内酰胺酶（ESBL）的分离株。此外，这些分离株对14种抗菌药物的耐药模式显示，大多数分离株对氨基糖苷类和β-内酰胺类抗生素耐药。TEM型基因在我们的分离株中普遍存在（79.17%）。根据ERIC-PCR结果，这些分离株（n = 48）被分为三个簇。发现了48种无重复的序列类型，揭示了分离株之间的遗传变异和相关性。因此，结果表明中国即食食品中存在产ESBL的情况。本研究结果为抗生素耐药菌株的传播提供了见解，并增进了对微生物风险的理解。
