Zhang Yue, Yao Duo, Huang Huan, Zhang Min, Sun Lina, Su Lin, Zhao LiHua, Guo Yueying, Jin Ye
College of Food Science and Engineering, Inner Mongolia Agricultural University, Hohhot 010018, China.
Integrative Research Base of Beef and Lamb Processing Technology, Ministry of Agriculture and Rural Affairs of the People's Republic of China, Hohhot 010018, China.
Food Sci Anim Resour. 2023 Sep;43(5):805-825. doi: 10.5851/kosfa.2023.e37. Epub 2023 Sep 1.
This experiment aims to investigate the impact of probiotic feed on growth performance, carcass traits, plasma lipid biochemical parameters, intramuscular fat and triglyceride content, fatty acid composition, mRNA expression levels of genes related to lipid metabolism, and the activity of the enzyme in Sunit sheep. In this experiment, 12 of 96 randomly selected Sunit sheep were assigned to receive the basic diet or the basic diet supplemented with probiotics. The results showed that supplementation with probiotics significantly increased the loin eye area, and decreased plasma triglycerides and free fatty acids, increasing the content of intramuscular fat and triglycerides in the muscle and improving the composition of the fatty acids. The inclusion of probiotics in the diet reduced the expression of adenosine 5'-monophosphate-activated protein kinase alpha 2 (AMPKα2) mRNA and carnitine palmitoyltransferase 1B (CPT1B) mRNA, while increasing the expression of acetyl-CoA carboxylase alpha (ACCα) mRNA, sterol regulatory element-binding protein-1c (SREBP-1c) mRNA, fatty acid synthase mRNA, and stearoyl-CoA desaturase 1 mRNA. The results of this study indicate that supplementation with probiotics can regulate fat deposition and improves the composition of fatty acids in Sunit sheep through the signaling pathways AMPK-ACC-CPT1B and AMPK-SREBP-1c. This regulatory mechanism leads to an increase in intramuscular fat content, a restructuring of muscle composition of the fatty acids, and an enhancement of the nutritional value of meat. These findings contribute to a better understanding of the food science of animal resources and provide valuable references for the production of meat of higher nutritional value.
本实验旨在研究益生菌饲料对苏尼特羊生长性能、胴体性状、血浆脂质生化参数、肌内脂肪和甘油三酯含量、脂肪酸组成、脂质代谢相关基因的mRNA表达水平以及酶活性的影响。在本实验中，从96只随机选取的苏尼特羊中挑选出12只，分别给予基础日粮或添加益生菌的基础日粮。结果表明，添加益生菌显著增加了眼肌面积，降低了血浆甘油三酯和游离脂肪酸水平，提高了肌肉中肌内脂肪和甘油三酯的含量，并改善了脂肪酸组成。日粮中添加益生菌降低了腺苷酸活化蛋白激酶α2（AMPKα2）mRNA和肉碱棕榈酰转移酶1B（CPT1B）mRNA的表达，同时增加了乙酰辅酶A羧化酶α（ACCα）mRNA、固醇调节元件结合蛋白-1c（SREBP-1c）mRNA、脂肪酸合酶mRNA和硬脂酰辅酶A去饱和酶1 mRNA的表达。本研究结果表明，添加益生菌可通过AMPK-ACC-CPT1B和AMPK-SREBP-1c信号通路调节苏尼特羊的脂肪沉积并改善脂肪酸组成。这种调节机制导致肌内脂肪含量增加、肌肉脂肪酸组成重构以及肉的营养价值提高。这些发现有助于更好地理解动物资源食品科学，并为生产具有更高营养价值的肉提供有价值的参考。
