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A型、B型和D型肉毒杆菌毒素可抑制牛肾上腺髓质细胞分泌儿茶酚胺。

Botulinum toxin types A, B and D inhibit catecholamine secretion from bovine adrenal medullary cells.

作者信息

Knight D E

出版信息

FEBS Lett. 1986 Oct 27;207(2):222-6. doi: 10.1016/0014-5793(86)81492-2.

DOI:10.1016/0014-5793(86)81492-2
PMID:3770197
Abstract

Evoked catecholamine secretion from cultured bovine adrenal medullary cells is inhibited by commercially available botulinum toxins - types A, B and D (10(4)-10(6) MLD/ml of culture medium). Basal secretion is also inhibited. The catecholamine content of such toxin-treated cells is larger than that of control cells and may in part be a result of the inhibition of basal release. The onset of action of botulinum toxin types A and D can be neutralised by their respective antisera. Concentrations of botulinum toxins A, B or D that inhibit secretion leave unaffected the 45Ca2+ influxes normally associated with secretion. These data provide further evidence to support the idea [(1985) Nature 317, 719-721] that botulinum toxins block secretion by acting downstream of the Ca2+ transient at or near the site of exocytosis.

摘要

市售的A型、B型和D型肉毒杆菌毒素（每毫升培养基含10⁴ - 10⁶个最小致死量）可抑制培养的牛肾上腺髓质细胞诱发的儿茶酚胺分泌。基础分泌也受到抑制。经此类毒素处理的细胞中儿茶酚胺含量高于对照细胞，这可能部分是基础释放受抑制的结果。A型和D型肉毒杆菌毒素的作用起始可被其各自的抗血清中和。抑制分泌的A型、B型或D型肉毒杆菌毒素浓度不影响通常与分泌相关的45Ca²⁺内流。这些数据为支持以下观点[(1985)《自然》317, 719 - 721]提供了进一步证据，即肉毒杆菌毒素通过在胞吐作用位点或其附近的Ca²⁺瞬变下游起作用来阻断分泌。

相似文献

1
Botulinum toxin types A, B and D inhibit catecholamine secretion from bovine adrenal medullary cells.A型、B型和D型肉毒杆菌毒素可抑制牛肾上腺髓质细胞分泌儿茶酚胺。
FEBS Lett. 1986 Oct 27;207(2):222-6. doi: 10.1016/0014-5793(86)81492-2.
2
Inhibition of exocytosis in bovine adrenal medullary cells by botulinum toxin type D.D型肉毒杆菌毒素对牛肾上腺髓质细胞胞吐作用的抑制
Nature. 1985;317(6039):719-21. doi: 10.1038/317719a0.
3
The effect of botulinum toxin type D on the triggered and constitutive exocytosis/endocytosis cycles in cultures of bovine adrenal medullary cells.D型肉毒杆菌毒素对牛肾上腺髓质细胞培养物中触发的和组成性的胞吐作用/内吞作用循环的影响。
FEBS Lett. 1992 Feb 24;298(2-3):118-22. doi: 10.1016/0014-5793(92)80035-f.
4
Calcium is released by exocytosis together with catecholamines from bovine adrenal medullary cells.钙与儿茶酚胺一起通过胞吐作用从牛肾上腺髓质细胞中释放出来。
J Neurochem. 1989 Aug;53(2):428-35. doi: 10.1111/j.1471-4159.1989.tb07352.x.
5
Distinct exocytotic responses of intact and permeabilised chromaffin cells after cleavage of the 25-kDa synaptosomal-associated protein (SNAP-25) or synaptobrevin by botulinum toxin A or B.肉毒杆菌毒素A或B切割25 kDa突触体相关蛋白（SNAP - 25）或突触小泡蛋白后，完整嗜铬细胞和透化嗜铬细胞的不同胞吐反应
Eur J Biochem. 1996 Mar 15;236(3):877-86. doi: 10.1111/j.1432-1033.1996.00877.x.
6
Reductive chain separation of botulinum A toxin--a prerequisite to its inhibitory action on exocytosis in chromaffin cells.肉毒杆菌A毒素的还原性链分离——其对嗜铬细胞胞吐作用产生抑制的前提条件。
FEBS Lett. 1989 May 8;248(1-2):23-7. doi: 10.1016/0014-5793(89)80424-7.
7
Intracellularly injected tetanus toxin inhibits exocytosis in bovine adrenal chromaffin cells.细胞内注射破伤风毒素可抑制牛肾上腺嗜铬细胞的胞吐作用。
Nature. 1986;324(6092):76-8. doi: 10.1038/324076a0.
8
Botulinum toxin-induced ADP-ribosylation and inhibition of exocytosis are unrelated events.肉毒杆菌毒素诱导的ADP核糖基化与胞吐作用的抑制是不相关的事件。
FEBS Lett. 1988 Oct 10;238(2):277-80. doi: 10.1016/0014-5793(88)80496-4.
9
Botulinum neurotoxin light chains inhibit both Ca(2+)-induced and GTP analogue-induced catecholamine release from permeabilised adrenal chromaffin cells.肉毒杆菌神经毒素轻链可抑制钙离子诱导的以及鸟苷三磷酸类似物诱导的儿茶酚胺从透化的肾上腺嗜铬细胞中释放。
FEBS Lett. 1996 May 20;386(2-3):137-40. doi: 10.1016/0014-5793(96)00432-2.
10
Botulinum A and the light chain of tetanus toxins inhibit distinct stages of Mg.ATP-dependent catecholamine exocytosis from permeabilised chromaffin cells.肉毒杆菌A和破伤风毒素轻链抑制来自透化嗜铬细胞的Mg.ATP依赖性儿茶酚胺胞吐作用的不同阶段。
Eur J Biochem. 1994 Jun 1;222(2):325-33. doi: 10.1111/j.1432-1033.1994.tb18871.x.

引用本文的文献

1
Future aspects of botulinum neurotoxins.肉毒杆菌神经毒素的未来发展方向。
J Neural Transm (Vienna). 2008;115(4):567-73. doi: 10.1007/s00702-007-0758-9. Epub 2007 Jun 8.
2
[Potential antinociceptive mechanisms of botulinum toxin].[肉毒杆菌毒素的潜在抗伤害感受机制]
Schmerz. 2006 Sep;20(5):381-2, 384-7. doi: 10.1007/s00482-006-0487-2.
3
Botulinum toxins--cause of botulism and systemic diseases?肉毒杆菌毒素——肉毒中毒及全身性疾病的病因？
Vet Res Commun. 2005 May;29(4):313-45. doi: 10.1023/b:verc.0000048489.45634.32.
4
A single amino acid near the C terminus of the synaptosomeassociated protein of 25 kDa (SNAP-25) is essential for exocytosis in chromaffin cells.25 kDa的突触体相关蛋白（SNAP - 25）C末端附近的单个氨基酸对于嗜铬细胞的胞吐作用至关重要。
Proc Natl Acad Sci U S A. 1999 Jun 22;96(13):7256-61. doi: 10.1073/pnas.96.13.7256.
5
Triggered exocytosis and endocytosis have different requirements for calcium and nucleotides in permeabilized bovine chromaffin cells.在通透的牛嗜铬细胞中，触发式胞吐作用和内吞作用对钙和核苷酸有不同的需求。
J Membr Biol. 1993 May;134(1):1-13. doi: 10.1007/BF00233471.
6
Evidence implicating protein kinase C in exocytosis from electropermeabilized bovine chromaffin cells.有证据表明蛋白激酶C参与电通透牛嗜铬细胞的胞吐作用。
J Membr Biol. 1988 Aug;104(1):21-34. doi: 10.1007/BF01871899.
7
Neurotransmitter release is blocked intracellularly by botulinum neurotoxin, and this requires uptake of both toxin polypeptides by a process mediated by the larger chain.肉毒杆菌神经毒素在细胞内阻断神经递质释放，这需要通过由较大链介导的过程摄取两种毒素多肽。
Proc Natl Acad Sci U S A. 1988 Jun;85(11):4090-4. doi: 10.1073/pnas.85.11.4090.
8
Distinct targets for tetanus and botulinum A neurotoxins within the signal transducing pathway in chromaffin cells.嗜铬细胞信号转导通路中破伤风毒素和肉毒杆菌A神经毒素的不同作用靶点。
Naunyn Schmiedebergs Arch Pharmacol. 1991 Oct;344(4):387-95. doi: 10.1007/BF00172577.