Adam-Vizi V, Rosener S, Aktories K, Knight D E
Division of Biomedical Sciences, King's College, London, England.
FEBS Lett. 1988 Oct 10;238(2):277-80. doi: 10.1016/0014-5793(88)80496-4.
The hypothesis that inhibition of secretion by botulinum neurotoxin type D occurs by an intracellular process involving ADP-ribosylation has been directly tested by measuring both the extent of inhibition of secretion and of ADP-ribosylation in the same cells. Although the inhibitory effect of unpurified toxin closely parallels intracellular ribosylation, the two events are clearly unrelated, as using purified D and C3 toxins together with their antibodies, each of these events can be either stimulated or inhibited independently of each other.
通过在同一细胞中测量分泌抑制程度和 ADP 核糖基化程度，直接检验了肉毒杆菌 D 型神经毒素通过涉及 ADP 核糖基化的细胞内过程抑制分泌这一假说。尽管未纯化毒素的抑制作用与细胞内核糖基化密切平行，但这两个事件显然无关，因为使用纯化的 D 型和 C3 毒素及其抗体，这些事件中的每一个都可以相互独立地被刺激或抑制。
