Shenzhen Key Laboratory of Smart Healthcare Engineering, Guangdong Provincial Key Laboratory of Advanced Biomaterials, Department of Biomedical Engineering, Southern University of Science and Technology, No. 1088 Xueyuan Rd, Nanshan District, Shenzhen, Guangdong 518055, China.
State Key Laboratory of Cardiovascular Disease, Fuwai Hospital, National Center for Cardiovascular Diseases, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100037, China.
Lab Chip. 2023 Sep 26;23(19):4324-4333. doi: 10.1039/d3lc00473b.
Particle separation plays a critical role in many biochemical analyses. In this article, we report a method of reverse flow enhanced inertia pinched flow fractionation (RF-iPFF) for particle separation. RF-iPFF separates particles by size based on the flow-induced inertial lift, and in the abruptly broadened segment, reverse flow is utilized to further enhance the separation distance between particles of different sizes. The separation performance can be significantly improved by reverse flow. Generally, compared with the case without reverse flow, this RF-iPFF technique can increase the particle throughput by about 10 times. To demonstrate the advantages of RF-iPFF, RF-iPFF was compared with traditional iPFF through a control experiment. RF-iPFF consistently outperformed iPFF across various conditions we studied. In addition, we use tumor cells spiked into the human whole blood to evaluate the separation performance of RF-iPFF.
粒子分离在许多生化分析中起着关键作用。本文报道了一种基于逆流增强惯性夹流分级（RF-iPFF）的粒子分离方法。RF-iPFF 根据流致惯性升力对粒子进行大小分离，在突然变宽的部分，利用逆流进一步增强不同大小粒子之间的分离距离。逆流可以显著提高分离性能。通常，与没有逆流的情况相比，这种 RF-iPFF 技术可以将粒子的通量提高约 10 倍。为了展示 RF-iPFF 的优势，我们通过对照实验将 RF-iPFF 与传统的 iPFF 进行了比较。在我们研究的各种条件下，RF-iPFF 始终优于 iPFF。此外，我们使用肿瘤细胞掺入人全血来评估 RF-iPFF 的分离性能。
