School of Science, Dalian Maritime University, Dalian, P. R. China.
College of Marine Engineering, Dalian Maritime University, Dalian, P. R. China.
Electrophoresis. 2021 Nov;42(21-22):2223-2229. doi: 10.1002/elps.202000325. Epub 2021 May 22.
To improve the accuracy and efficiency of ships' ballast water detection, the separation of microalgae according to size is significant. In this article, a method to separate microalgae based on inertia-enhanced pinched flow fractionation (iPFF) was reported. The method utilized the inertial lift force induced by flow to separate microalgae according to size continuously. The experimental results show that, as the Reynolds number increases, the separation effect becomes better at first, but then stays unchanged. The best separation effect can be obtained when the Reynolds number is 12.3. In addition, with the increase of the flow rate ratio between sheath fluid and microalgae mixture, the separation effect becomes better and the best separation effect can be obtained when the flow rate ratio reaches 10. In this case, the recovery rate of Tetraselmis sp. is about 90%, and the purity is about 86%; the recovery rate of Chlorella sp. is as high as 99%, and the purity is about 99%. After that, the separation effect keeps getting better but very slowly. In general, this study provides a simple method for the separation of microalgae with different sizes, and lays a foundation for the accurate detection of microalgae in the ballast water.
为了提高船舶压载水检测的准确性和效率，根据尺寸对微藻进行分离是很重要的。本文报道了一种基于惯性增强型变形流分级（iPFF）的微藻分离方法。该方法利用流场产生的惯性升力，连续地根据尺寸对微藻进行分离。实验结果表明，随着雷诺数的增加，分离效果先变好后不变，当雷诺数为 12.3 时，分离效果最佳。此外，随着鞘液与微藻混合物流速比的增加，分离效果变好，当流速比达到 10 时，分离效果最佳。在此条件下，三角褐指藻的回收率约为 90%，纯度约为 86%；小球藻的回收率高达 99%，纯度约为 99%。之后，分离效果虽仍在变好，但非常缓慢。总的来说，本研究为不同尺寸的微藻分离提供了一种简单的方法，为压载水中微藻的准确检测奠定了基础。
