Miyazaki S, Hashimoto N, Yoshimoto Y, Kishimoto T, Igusa Y, Hiramoto Y
Dev Biol. 1986 Nov;118(1):259-67. doi: 10.1016/0012-1606(86)90093-x.
A series of periodic increases in intracellular free calcium concentration ([Ca2+]i) occurred upon fertilization in golden hamster eggs. The spatial distribution of the Ca2+ transients was investigated in single zona-free, aequorin-injected eggs, inseminated by single sperm. A supersensitive TV camera system for recording Ca2+-aequorin luminescence enabled us to observe the spatial distribution of the Ca2+ rise. In the first response, which usually occurred 10-30 sec after the sperm attachment, the increase in [Ca2+]i began near the sperm attachment site, and the Ca2+ rise spread over the entire egg within 4-7 sec. The Ca2+ rise attained its peak in 5-8 sec, declined with almost even distribution, and ceased in 12-17 sec. The spreading Ca2+ rise was repeated in the second and sometimes the third response, starting from the same focus, but spreading more rapidly (approximately 2 sec). In succeeding responses [Ca2+]i increased synchronously in the whole cytoplasm within 1 sec. When additional sperm attached to the egg after the occurrence of the first response by the first sperm, the spread of the Ca2+ rise could take place from near the site of additional sperm attachment but only in the second or third response.
金黄地鼠卵受精后，细胞内游离钙浓度（[Ca2+]i）出现一系列周期性升高。在单个去透明带、注射了水母发光蛋白的卵中研究了Ca2+瞬变的空间分布，这些卵由单个精子受精。用于记录Ca2+-水母发光蛋白发光的超灵敏电视摄像系统使我们能够观察到Ca2+升高的空间分布。在通常在精子附着后10-30秒发生的第一次反应中，[Ca2+]i的升高始于精子附着部位附近，Ca2+升高在4-7秒内扩散到整个卵。Ca2+升高在5-8秒达到峰值，以几乎均匀的分布下降，并在12-17秒停止。从同一焦点开始，在第二次反应中有时在第三次反应中会重复Ca2+升高的扩散，但扩散速度更快（约2秒）。在随后的反应中，[Ca2+]i在1秒内在整个细胞质中同步升高。当第一个精子发生第一次反应后，额外的精子附着在卵上时，Ca2+升高的扩散可以从额外精子附着部位附近开始，但仅在第二次或第三次反应中发生。
