Zhou Mengyuan, Qiang Jingchao, Gan Jiajie, Xu Xuhui, Li Xing, Zhang Shuai, Xu Baoshi, Dong Zibo
Jiangsu Key Laboratory of Marine Bioresources and Environment, Co-Innovation Center of Jiangsu Marine Bio-industry Technology, Jiangsu Key Laboratory of Marine Pharmaceutical Compound Screening, College of Pharmacy, Jiangsu Ocean University, Lianyungang 222005, China.
Jiangsu Key Laboratory of Marine Bioresources and Environment, Co-Innovation Center of Jiangsu Marine Bio-industry Technology, Jiangsu Key Laboratory of Marine Pharmaceutical Compound Screening, College of Pharmacy, Jiangsu Ocean University, Lianyungang 222005, China.
Comp Biochem Physiol C Toxicol Pharmacol. 2023 Dec;274:109744. doi: 10.1016/j.cbpc.2023.109744. Epub 2023 Sep 12.
Avermectin (AVM) is one of the most often used insecticides which is toxic to aquatic organisms, and cause oxidative-induced damages to the fish respiratory organ, the "gills". To better understand the mechanism by which an antioxidant reduces AVM-induced gill damage, we investigated the effects of Quercetin (Que) on AVM induction of oxidative stress to inhibit damages to the gills using common carp as a model organism. The Que is a fruit and vegetable rich flavonoid with antioxidant activity. In this study, four groups were created: the Control group, the Que group (400 mg/kg), the AVM group (2.404 μg/L), and the Que plus AVM group. The analytical methods were pathological structure examination, qPCR, Reactive Oxygen Species (ROS) and Western blot. The results showed that Que alleviated AVM-induced oxidative stress, inflammatory damage and apoptosis in the carp gills by activating the Nrf2 pathway. The mechanism was that Que alleviated the accumulation of ROS, reduced the balance between oxidation and antioxidant disrupted by AVM exposure, lowered the content of lipid peroxidation produced malondialdehyde (MDA), and increased the content of antioxidant enzymes including glutathione (GSH) and catalase (CAT). Nrf2 pathway was activated. Meanwhile, Que inhibited gill apoptosis in carp by decreasing the levels of Bax, Cytochrome C, Caspase9, Cleaved-Caspase3 and reduced Bcl2. This has important implications for future studies on Que and AVM. New suggestions are provided to reduce the threat of aquatic environmental pollution.
阿维菌素（AVM）是最常用的杀虫剂之一，对水生生物有毒，并会对鱼类呼吸器官“鳃”造成氧化诱导损伤。为了更好地理解抗氧化剂减轻AVM诱导的鳃损伤的机制，我们以鲤鱼为模式生物，研究了槲皮素（Que）对AVM诱导的氧化应激以抑制鳃损伤的影响。Que是一种富含抗氧化活性的果蔬类黄酮。在本研究中，设置了四组：对照组、Que组（400mg/kg）、AVM组（2.404μg/L）和Que加AVM组。分析方法包括病理结构检查、qPCR、活性氧（ROS）检测和蛋白质免疫印迹法。结果表明，Que通过激活Nrf2通路减轻了AVM诱导的鲤鱼鳃氧化应激、炎症损伤和细胞凋亡。其机制是Que减轻了ROS的积累，恢复了因暴露于AVM而被破坏的氧化与抗氧化之间的平衡，降低了脂质过氧化产生的丙二醛（MDA）含量，并增加了包括谷胱甘肽（GSH）和过氧化氢酶（CAT）在内的抗氧化酶含量，从而激活了Nrf2通路。同时，Que通过降低Bax、细胞色素C、Caspase9、裂解的Caspase3水平并上调Bcl2，抑制了鲤鱼鳃细胞凋亡。这对未来关于Que和AVM的研究具有重要意义。为减少水生环境污染威胁提供了新的建议。
