Koike T I, Neldon H L, McKay D W, Rayford P L
Gen Comp Endocrinol. 1986 Jul;63(1):93-103. doi: 10.1016/0016-6480(86)90186-3.
This report presents characteristics of an antiserum raised in a rabbit immunized with synthetic mesotocin (MT) conjugated to bovine thyroglobulin. Cross-reactivity studies indicate that the antiserum (Kl-II) recognizes the carboxyl-terminal "tail" of MT and isotocin (IT). A homologous, disequilibrium radioimmunoassay (RIA) for MT has been developed that can detect less than 1 pg of peptide. Plasma was extracted with octadecasilyl-silica. Recovery of MT from plasma was correlated with the amount added and averaged 70%. Different volumes of plasma and posterior pituitary extract, when measured in the assay system, yielded inhibition curves that were parallel with standard MT. Immunoreactive MT and AVT of plasma and neural lobe coeluted with synthetic standards after gel filtration. The ED50 of a heterologous, sequential saturation RIA for IT was 17.4 pg, suggesting that the MT antiserum may be useful for measuring the oxytocin-like principle in bony fishes. Immunoreactive MT in plasma of cockerels increased and decreased with iv infusion of hypo- and hyperosmotic saline, respectively. The changes in plasma MT were inversely related to osmolality. Hyperosmotic saline infusion resulted in correlated increases in plasma AVT and osmolality. The data suggest that MT may be released by dilution and/or expansion of extracellular fluid in chickens.
本报告介绍了用与牛甲状腺球蛋白偶联的合成促黑素细胞激素（MT）免疫家兔产生的抗血清的特性。交叉反应研究表明，该抗血清（Kl-II）识别MT和异促黑素细胞激素（IT）的羧基末端“尾”。已开发出一种用于MT的同源、非平衡放射免疫分析（RIA），可检测到少于1 pg的肽。血浆用十八烷基硅烷硅胶提取。MT从血浆中的回收率与添加量相关，平均为70%。在分析系统中测量不同体积的血浆和垂体后叶提取物时，得到的抑制曲线与标准MT平行。凝胶过滤后，血浆和神经叶的免疫反应性MT和精氨酸血管加压素（AVT）与合成标准品共洗脱。IT的异源顺序饱和RIA的半数有效剂量（ED50）为17.4 pg，这表明MT抗血清可能有助于测量硬骨鱼中的催产素样物质。分别静脉输注低渗和高渗盐水时，公鸡血浆中的免疫反应性MT含量相应降低和升高。血浆MT的变化与渗透压呈负相关。输注高渗盐水导致血浆AVT和渗透压相关升高。数据表明，MT可能通过鸡细胞外液的稀释和/或扩张而释放。
