miR-34a 在儿童肺炎支原体肺炎中的诊断价值及其与康复效果的相关性。

Diagnostic value of miR-34a in Mycoplasma pneumoniae pneumonia in children and its correlation with rehabilitation effect.

机构信息

Department of Pediatrics, Affiliated Haimen Hospital of Xinglin College, Nantong University, Nantong, 226100, China.

Department of Rehabilitation, Children's Hospital of Nanjing Medical University, No. 72 Guangzhou Road, Nanjing, Jiangsu Province, 210000, China.

出版信息

J Cardiothorac Surg. 2024 Sep 2;19(1):507. doi: 10.1186/s13019-024-02992-5.

DOI:10.1186/s13019-024-02992-5

PMID:39223566

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11367975/

Abstract

BACKGROUND

Mycoplasma pneumoniae pneumonia (MPP) is responsible for 20 to 40% of all cases of pneumonia acquired by children and shows an increasing incidence year by year. The aim of this study was to investigate the expression of miR-34a in children with MPP and its diagnostic value, and further explore the relationship between miR-34a and the rehabilitation effect of children with MPP.

METHODS

The expression level of miR-34a was detected by RT-qPCR, and the clinical value of miR-34a was analyzed by ROC analysis. In addition, the levels of IL-6, IL-18 and TNF-α in serum of children with MPP were detected by ELISA kit, and the correlation with miR-34a was analyzed.

RESULTS

Elevated levels of miR-34a were observed in the serum of children with MPP, and significantly higher expression levels were observed in children with severe symptoms and poor rehabilitation. The study suggested that miR-34a has potential as a diagnostic marker for MPP in children, helping to distinguish between mild and severe cases and predicting rehabilitation from MPP in children. In addition, miR-34a expression was positively correlated with IL-6, IL-8, and TNF-α levels.

CONCLUSIONS

miR-34a is closely related to MPP in children and miR-34a may be used as a clinical biomarker for MPP in children.

摘要

背景

肺炎支原体肺炎（MPP）占儿童获得性肺炎的 20%至 40%，且发病率呈逐年上升趋势。本研究旨在探讨 miR-34a 在儿童 MPP 中的表达及其诊断价值，并进一步探讨 miR-34a 与儿童 MPP 康复效果的关系。

方法

采用 RT-qPCR 检测 miR-34a 的表达水平，通过 ROC 分析分析 miR-34a 的临床价值。此外，采用 ELISA 试剂盒检测儿童 MPP 血清中 IL-6、IL-18 和 TNF-α的水平，并分析与 miR-34a 的相关性。

结果

儿童 MPP 血清中 miR-34a 水平升高，症状严重和康复不良的儿童表达水平显著升高。研究表明，miR-34a 具有作为儿童 MPP 的潜在诊断标志物的潜力，有助于区分轻度和重度病例，并预测儿童 MPP 的康复情况。此外，miR-34a 的表达与 IL-6、IL-8 和 TNF-α水平呈正相关。