利用 SucB 重组抗原检测反刍动物中的抗体。
Detection of antibodies in ruminants using a SucB recombinant antigen.
机构信息
Department of Veterinary Medicine and Animal Productions, University of Naples, "Federico II", Naples, Italy.
Department of Veterinary Science, University of Turin, Grugliasco, TO, Italy.
出版信息
J Vet Diagn Invest. 2023 Nov;35(6):721-726. doi: 10.1177/10406387231199964. Epub 2023 Sep 13.
The detection of in ruminants remains challenging despite the use of new technology and the accumulation of novel knowledge. Serology tools, the primary methods of infection surveillance in veterinary medicine, have limitations. We used recombinant antigen production to develop an ELISA based on the SucB protein, one of the major immunodominant antigens described in humans and laboratory animals. We produced the antigen successfully in an heterologous system, confirmed by sequencing and mass spectrometry, and seen as a band of ~50 kDa in SDS-PAGE and on western blot analysis. We compared the performance of the recombinant ELISA with a commercial ELISA. We observed agreement of 83.5% and a substantial Cohen κ value of 0.67 in our pilot study.
尽管新技术的应用和新的知识积累，反刍动物中的 的检测仍然具有挑战性。血清学工具是兽医感染监测的主要方法，但存在局限性。我们使用重组抗原生产技术，基于 SucB 蛋白开发了一种 ELISA，该蛋白是在人类和实验动物中描述的主要免疫显性抗原之一。我们成功地在异源系统中生产了该抗原，通过测序和质谱分析得到了确认，并在 SDS-PAGE 和 Western blot 分析中观察到约 50 kDa 的条带。我们比较了重组 ELISA 和商业 ELISA 的性能。在我们的初步研究中，观察到了 83.5%的一致性和 0.67 的显著 Cohen κ 值。
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