State Key Laboratory of Animal Nutrition, Key Laboratory of Animal (Poultry) Genetics Breeding and Reproduction, Ministry of Agriculture, Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing, China.
Institute of Animal Husbandry and Veterinary Medicine, Zhejiang Academy of Agricultural Sciences, Hangzhou, China.
Anim Genet. 2023 Dec;54(6):803-807. doi: 10.1111/age.13358. Epub 2023 Sep 13.
Semen is a measure of the reproductive efficiency of roosters, which affects the economic benefits of white-feathered broilers. Over the years, research in this field has mainly focused on hens, while there have been fewer studies on the reproductive traits of roosters. To identify the genes related to the semen traits of roosters, we used a chicken 55 K SNP chip to genetically type the white-feathered population (220) and performed imputation with resequencing data from 97 roosters. In total, 1 048 576 SNPs were obtained and used for genome-wide association analysis of semen volume, from which 197 genome-wide significant markers were identified, all within the interval of 13.82-16.12 Mb on chromosome 7. By combining our results with the biological functions of genes in the interval, four candidate genes were identified that potentially relate to semen volume: FAPP1, OSBPL6, SESTD1 and SSFA2. Our findings may provide a basis for further research on the genetic mechanism and marker-assisted selection of semen volume in white-feathered broilers.
精液是衡量公鸡繁殖效率的指标，它影响白羽肉鸡的经济效益。多年来，该领域的研究主要集中在母鸡上，而对公鸡繁殖特性的研究较少。为了鉴定与公鸡精液特性相关的基因，我们使用鸡 55K SNP 芯片对白羽群体（220 个）进行基因分型，并利用 97 只公鸡的重测序数据进行了 imputation。总共获得了 1048576 个 SNPs，并用于精液量的全基因组关联分析，从中鉴定出 197 个全基因组显著标记，均位于第 7 号染色体的 13.82-16.12Mb 区间内。通过将我们的结果与该区间内基因的生物学功能相结合，鉴定出了四个可能与精液量相关的候选基因：FAPP1、OSBPL6、SESTD1 和 SSFA2。我们的研究结果可能为进一步研究白羽肉鸡精液量的遗传机制和标记辅助选择提供依据。
