Greenway D C, Nanji A A
Gynecol Obstet Invest. 1986;22(2):108-9. doi: 10.1159/000298900.
A digoxin-like immunoreactive substance (DLIS) has been reported in the amniotic fluid. Since radioimmunoassay kits are standardized using serum-based standards, we hypothesized that measurement of DLIS may be an artifact related to the low protein content of amniotic fluid. We analyzed 12 amniotic fluid samples before and after supplementation with lyophilized human serum. The means +/- SDs for DLIS (nmol/l) at protein concentrations of 0, 32 and 63 g/l were 1.4 +/- 0.16, 0.6 +/- 0.09, and 0.4 +/- 0.09 nmol/l, respectively. We, therefore, hypothesize that DLIS in amniotic fluid may in part be explained by a technical artifact.
羊水内已报道有洋地黄样免疫反应物质（DLIS）。由于放射免疫分析试剂盒是使用基于血清的标准品进行标准化的，我们推测DLIS的测量可能是与羊水低蛋白含量相关的一种假象。我们分析了12份羊水样本，在补充冻干人血清前后的情况。蛋白质浓度为0、32和63 g/l时，DLIS（nmol/l）的均值±标准差分别为1.4±0.16、0.6±0.09和0.4±0.09 nmol/l。因此，我们推测羊水内的DLIS部分可能由技术假象所解释。
