Guangdong Provincial Key Laboratory of Large Animal Models for Biomedicine, South China Institute of Large Animal Models for Biomedicine, School of Biotechnology and Health Science, Wuyi University, Jiangmen, China.
International Healthcare Innovation Institute (Jiangmen), Jiangmen, China.
Clin Genet. 2023 Dec;104(6):613-624. doi: 10.1111/cge.14424. Epub 2023 Sep 14.
Cancer, one of the leading causes of death, usually commences and progresses as a result of a series of gene mutations and dysregulation of expression. With the development of clustered regularly interspaced palindromic repeat (CRISPR)/Cas9 gene-editing technology, it is possible to edit and then decode the functions of cancer-related gene mutations, markedly advance the research of biological mechanisms and treatment of cancer. This review summarizes the mechanism and development of CRISPR/Cas9 gene-editing technology in recent years and describes its potential application in cancer-related research, such as the establishment of human tumor disease models, gene therapy and immunotherapy. The challenges and future development directions are highlighted to provide a reference for exploring pathological mechanisms and potential treatment protocols of cancer.
癌症是导致死亡的主要原因之一，通常是由于一系列基因突变和表达失调而引发和进展的。随着成簇规律间隔短回文重复序列 (CRISPR)/Cas9 基因编辑技术的发展，可以对与癌症相关的基因突变进行编辑和解码，从而显著推进癌症的生物学机制和治疗研究。本综述总结了近年来 CRISPR/Cas9 基因编辑技术的机制和发展，并描述了其在癌症相关研究中的潜在应用，如建立人类肿瘤疾病模型、基因治疗和免疫治疗。本文强调了该技术面临的挑战和未来发展方向，为探索癌症的病理机制和潜在治疗方案提供了参考。
