Doctoral School of the Rzeszów University of Technology, Rzeszów, Poland.
Faculty of Chemistry, Rzeszów University of Technology, Rzeszów, Poland.
Rapid Commun Mass Spectrom. 2023 Oct 30;37(20):e9621. doi: 10.1002/rcm.9621.
Hormones are compounds that perform many important functions in the human body, but above all their task is to maintain homeostasis by adapting them to the constantly changing environmental conditions. Even minor hormonal disorders have a negative effect on the body, leading to physical or mental changes. Therefore, monitoring these changes and precise quantification of hormones are essential for the early diagnosis of diseases related to hormonal disorders.
Application of monoisotopic silver-109 and gold nanoparticles obtained by PFL (pulsed fiber laser) 2D GS (galvo-scanner) LGN (laser-generated nanomaterial) for high-resolution laser desorption/ionization mass spectrometry (LDI-MS) and mass spectrometry imaging (MSI) of steroid hormones is presented. Four steroid hormones, estrone, prednisolone, corticosterone and progesterone, were used as test compounds for quantitative analysis with matrix-assisted LDI time-of-flight MS apparatus. Moreover, comparison of manual measurements and semiautomatic MSI with both types of nanoparticles was performed. Methods were also tested on spiked human blood serum for quantification of steroid hormones and for estimation of the matrix effect.
Hormones were directly tested in 1 000 000-fold concentration change conditions ranging from 1 mg/mL to 1 ng/mL which equates to 300 ng to 300 fg of hormone per measurement spot. For almost all tested hormones MSI allowed one to obtain equal or lower limit of detection value than manual LDI-MS. The best results judged by lowest limit of detection values are found for silver-109 nanoparticles.
The results of the quantitative analysis of steroid hormones using silver-109 and gold nanoparticles prepared with PFL 2D GS LGN for LDI-MS and semiautomatic LDI-MSI are presented. It has been proven that nanoparticles obtained by laser synthesis can be successfully used for the analysis of steroid hormones in a wide range of concentrations.
激素是在人体内发挥许多重要功能的化合物，但它们的主要任务是通过适应不断变化的环境条件来维持体内平衡。即使是轻微的激素紊乱也会对身体产生负面影响，导致身体或精神上的变化。因此，监测这些变化和激素的精确定量对于早期诊断与激素紊乱相关的疾病至关重要。
介绍了应用单同位素银-109 和金纳米粒子，通过 PFL（脉冲光纤激光）2D GS（振镜）LGN（激光产生的纳米材料）进行类固醇激素的高分辨率激光解吸/电离质谱（LDI-MS）和质谱成像（MSI）。使用雌酮、泼尼松龙、皮质酮和孕酮这四种甾体激素作为测试化合物，用基质辅助 LDI 飞行时间 MS 仪器进行定量分析。此外，还对两种类型的纳米粒子的手动测量和半自动化 MSI 进行了比较。还对加标人血清中的激素进行了方法测试，以定量测定甾体激素并估计基质效应。
在 1 000 000 倍浓度变化范围内（从 1 mg/mL 到 1 ng/mL）直接测试了激素，相当于每个测量点有 300 ng 到 300 fg 的激素。对于几乎所有测试的激素，MSI 都可以获得与手动 LDI-MS 相等或更低的检测限。根据最低检测限值判断，银-109 纳米粒子的结果最佳。
介绍了使用 PFL 2D GS LGN 制备的银-109 和金纳米粒子进行 LDI-MS 和半自动 LDI-MSI 对甾体激素进行定量分析的结果。已经证明，激光合成得到的纳米粒子可以成功地用于分析各种浓度的甾体激素。
