红外脉冲光纤激光产生的银-109 纳米颗粒用于 3-羟基羧酸的激光解吸/电离质谱。

Infrared pulsed fiber laser-produced silver-109 nanoparticles for laser desorption/ionization mass spectrometry of 3-hydroxycarboxylic acids.

机构信息

Doctoral School of Engineering and Technical Sciences at the Rzeszów University of Technology, Rzeszów, Poland.

Faculty of Chemistry, Rzeszów University of Technology, Rzeszów, Poland.

出版信息

Rapid Commun Mass Spectrom. 2022 Nov 15;36(21):e9375. doi: 10.1002/rcm.9375.

DOI:10.1002/rcm.9375

PMID:35933593

Abstract

RATIONALE

3-Hydroxycarboxylic acids are one of the major components of bacterial lipopolysaccharides (LPS), also known as endotoxins. Endotoxins pose a serious health risk and can seriously damage the internal organs of humans and animals. 3-Hydroxycarboxylic acids can be used as environmental markers to determine endotoxin levels. At the time of preparation of this manuscript no studies on laser mass spectrometry (MS) and analysis with silver nanoparticles (NP) for 3-hydroxycarboxylic acids have been published in literature.

METHODS

Six acids, 3-hydroxyoctanoic (3-OH-C8:0), 3-hydroxydecanoic (3-OH-C10:0), 3-hydroxydodecanoic (3-OH-C12:0), 3-hydroxytetradecanoic (3-OH-C14:0), 3-hydroxyhexadecanoic (3-OH-C16:0), and 3-hydroxyoctadecanoic (3-OH-C18:0) acids, were used as test compounds on the target containing silver-109 NPs for quantification using matrix-assisted laser desorption/ionization (MALDI)-type mass spectrometer. Methods were also tested on spiked human blood serum samples to quantify 3-hydroxycarboxylic acids and verify the influence of the biological matrix on the measurement.

RESULTS

Analyzed acids were directly tested in 1 000 000-fold concentration change conditions ranging from 1 mg/mL to 1 ng/mL. The semi-automatic MSI (MS imaging) method allowed us to obtain two to five times lower limit of detection (LOD) and lower limit of quantitation (LLOQ) values than common LDI (Bruker Daltonics, Bremen, Germany) method for analyzed acids. For almost all results of 3-hydroxycarboxylic acids, the trendline fit was better for the semi-automatic MSI method than the manual LDI method.

CONCLUSION

For the first time, the use of laser MS for the quantification of 3-hydroxycarboxylic acids has been demonstrated, and it has been proven that it can be used in the quantitative analysis of such compounds over a wide range of concentrations. In addition, a comparison of two methods-manual LDI-MS and semi-automatic MSI-is presented.

摘要

原理

3-羟基羧酸是细菌脂多糖（LPS）的主要成分之一，也称为内毒素。内毒素对健康构成严重威胁，可严重损害人类和动物的内脏器官。3-羟基羧酸可用作环境标志物来确定内毒素水平。在撰写本文时，尚无关于激光质谱（MS）和用银纳米粒子（NP）分析 3-羟基羧酸的研究发表。

方法

六种酸，即 3-羟基辛酸（3-OH-C8:0）、3-羟基癸酸（3-OH-C10:0）、3-羟基十二酸（3-OH-C12:0）、3-羟基十四酸（3-OH-C14:0）、3-羟基十六酸（3-OH-C16:0）和 3-羟基十八酸（3-OH-C18:0），被用作含有银-109NP 的目标化合物的测试化合物，用于使用基质辅助激光解吸/电离（MALDI）-MS 进行定量。还在加标人血清样本上测试了方法，以定量 3-羟基羧酸并验证生物基质对测量的影响。

结果

在 1mg/mL 至 1ng/mL 的浓度变化条件下，直接测试了分析用酸。半自动 MSI（MS 成像）方法使我们能够获得比常规 LDI（德国不来梅的 Bruker Daltonics）方法低 2 至 5 倍的检测限（LOD）和定量限（LLOQ）值。对于分析用酸的几乎所有结果，半自动 MSI 方法的趋势线拟合都优于手动 LDI 方法。