Department of Chemistry, Division of Biology (Microbiology), University College of Qunfudah, Umm Al-Qura University, Qunfudah, Saudi Arabia.
Botany and Microbiology Department, Faculty of Science, Zagazig University, Zagazig, Egypt; Biology Department, Faculty of Science and Arts in Al-Mikhwah, Al-Baha University, Al-Mikhwah, Saudi Arabia.
Microb Pathog. 2023 Nov;184:106343. doi: 10.1016/j.micpath.2023.106343. Epub 2023 Sep 12.
Agriculture activities industries produce a huge amount of waste every year. Agricultural wastes are a great source of natural polysaccharides characterized by accessibility, biocompatibility, and ease of modification. Finding new safe antibacterial agents has become one of the top priorities of health organizations worldwide. This priority emerged from the antibiotic resistance pathogenic bacteria hazard. Carcinogenic bacteria are one of the most dangerous antibiotic-resistant pathogenic bacteria. This study tries to investigate the antibacterial activity of polysaccharides from some agricultural wastes against carcinogenic bacteria related to gastrointestinal cancers. We determined the antibacterial activity (in terms of minimum inhibitory concentration (MIC)) and the biofilm reduction capacity. We studied the mechanism of the antibacterial activity by determining the effect of the MIC of the extracted polysaccharides on the plasma membrane permeability and the bacterial DNA content. All extracted polysaccharides showed effective antibacterial activity with low MICs ranging from 2 to 20 μg/mL. The barely straw polysaccharides showed the highest MIC (19.844 μg/mL) against Bacteroides fragilis, while the grape bagasse showed the lowest MIC (2.489 μg/mL) against Helicobacter pylori. The extracted polysaccharide showed high antibiofilm activity. Their capacity to reduce the formation of the pathogenic biofilm ranged from 75 to 95%. Regarding the antibacterial mechanism, the extracted polysaccharides showed destructive action on the DNA and the plasma membrane permeability. The bacterial DNA change percent after the treatment with the different polysaccharides ranged from 29% to -58%. The plasma membrane permeability increased by a high percentage, ranging from 92% to 123%. Agricultural waste polysaccharides are a promising antibacterial agent against antibiotic-resistant carcinogenic bacteria.
农业活动产业每年产生大量的废物。农业废物是天然多糖的重要来源，其特点是易得、生物相容性好且易于修饰。寻找新的安全抗菌剂已成为全球卫生组织的首要任务之一。这一优先事项源于对抗生素耐药病原菌的危害。致癌细菌是最危险的抗生素耐药病原菌之一。本研究试图研究一些农业废物中的多糖对与胃肠道癌症相关的致癌细菌的抗菌活性。我们确定了抗菌活性（以最小抑菌浓度 (MIC) 表示）和生物膜减少能力。我们通过确定提取多糖的 MIC 对质膜通透性和细菌 DNA 含量的影响来研究抗菌活性的机制。所有提取的多糖都表现出有效的抗菌活性，MIC 低至 2 至 20 μg/mL。几乎没有草多糖对脆弱拟杆菌的 MIC 最高（19.844 μg/mL），而葡萄渣对幽门螺杆菌的 MIC 最低（2.489 μg/mL）。提取的多糖表现出高的抗生物膜活性。它们抑制病原菌生物膜形成的能力在 75%至 95%之间。关于抗菌机制，提取的多糖对 DNA 和质膜通透性表现出破坏作用。用不同多糖处理后，细菌 DNA 的变化百分比在 29%至-58%之间。质膜通透性增加了很大的百分比，范围从 92%到 123%。农业废物多糖是一种有前途的对抗生素耐药致癌细菌的抗菌剂。
