Department of Medical Sciences, College of Medical Sciences, Soonchunhyang University, Asan, Republic of Korea.
Department of Biomedical Laboratory Science, College of Health Sciences, Yonsei University at Wonju, Wonju 26493, Republic of Korea.
Can J Microbiol. 2020 Jun;66(6):389-399. doi: 10.1139/cjm-2020-0004. Epub 2020 Feb 19.
The rapid increase in antibiotic resistance has prompted the discovery of drugs that reduce antibiotic resistance or new drugs that are an alternative to antibiotics. Plant extracts have health benefits and may also exhibit antibacterial and antibiofilm activities against pathogens. This study determined the antibacterial and antibiofilm effects of α-humulene extracted from plants against enterotoxigenic , which causes inflammatory bowel disease. The minimum inhibitory concentration and biofilm inhibitory concentration of α-humulene for were 2 μg/mL, and the biofilm eradication concentration was in the range of 8-32 μg/mL. The XTT reduction assay confirmed that the cellular metabolic activity in biofilm rarely occurred at the concentration of 8-16 μg/mL. In addition, biofilm inhibition by α-humulene was also detected via confocal laser scanning microcopy. Quantitative real-time polymerase chain reaction (qPCR) was also used to investigate the effect of α-humulene on the expression of resistance-nodulation-cell division type multidrug efflux pump genes ( and ). According to the results of qPCR, α-humulene significantly reduced the expression of and genes. This study demonstrates the potential therapeutic application of α-humulene for inhibiting the growth of cells and biofilms, and it expands the knowledge about biofilm medicine.
抗生素耐药性的迅速增加促使人们发现了能够降低抗生素耐药性的药物或替代抗生素的新药。植物提取物对健康有益，并且可能对病原体具有抗菌和抗生物膜活性。本研究测定了植物中提取的 α-葎草烯对引起炎症性肠病的肠毒素产生菌的抗菌和抗生物膜作用。α-葎草烯对 的最小抑菌浓度和生物膜抑制浓度为 2μg/mL，生物膜清除浓度在 8-32μg/mL 范围内。XTT 还原测定证实，在 8-16μg/mL 的浓度下，生物膜中细胞代谢活性很少发生。此外，还通过共聚焦激光扫描显微镜检测到 α-葎草烯对生物膜的抑制作用。还使用定量实时聚合酶链反应 (qPCR) 研究了 α-葎草烯对耐药性-结节-细胞分裂型多药外排泵基因 ( 和 ) 表达的影响。根据 qPCR 的结果，α-葎草烯显著降低了 和 基因的表达。本研究证明了 α-葎草烯抑制 细胞生长和生物膜的潜在治疗应用，并扩展了生物膜医学的知识。
