Department of Biotechnology and Biomedicine, Technical University of Denmark, Lyngby, Denmark.
Research Center for Micro-Ecological Agent Engineering and Technology of Guangdong Province, Guangzhou, Guangdong Province, China.
J Glob Antimicrob Resist. 2023 Dec;35:149-158. doi: 10.1016/j.jgar.2023.08.020. Epub 2023 Sep 13.
The various sequence types (STs) of Pseudomonas aeruginosa (P. aeruginosa) high-risk clones (HiRiCs) have been sporadically reported in China, but the systematic analysis of genomes for these STs remains limited. This study aimed to address the evolutionary pathways underlying the emergence of HiRiCs and their routes of dissemination from Chinese and global perspectives.
The phylogenetic analysis was performed based on 416 newly sequenced clinical P. aeruginosa strains from Guangdong (GD), published genome sequences of 282 Chinese isolates, and 868 HiRiCs isolates from other countries. The genomic comparison study of global HiRiC ST244 was conducted to detect the model of global dissemination and local separation driven by association regional-specific antibiotic resistance genes. Furthermore, the evolutionary route of the emerging, China-specific HiRiC ST1971 was explored using Most Recent Common Ancestor (MRCA) analysis.
Based on comparative genomics analysis, we found a clear geographical separation of ST244 isolates, yet with an association between ST244 isolates from GD and America. We identified a set of 38 AMR genes that contribute to the geographical separation in ST244, and we identified genetic determinants either positively (MexB) and negatively (opmD) associated with GD ST244. For the China-unique HiRiC ST1971, its evolutionary history across different continents before emerging as ST1971 in China was also deduced.
This study provides insight into the specific genetics underlying regional differences among globally disseminated P. aeruginosa HiRiCs (ST244) as well as new understanding of the dissemination and evolution of a regional HiRiC (ST1971). Understanding the genetics of these and other HiRiCs may assist in controlling their emergence and further spread.
铜绿假单胞菌（P. aeruginosa）高危克隆（HiRiC）的各种序列型（ST）在中国时有报道，但对这些 ST 基因组的系统分析仍然有限。本研究旨在从中国和全球角度分析 HiRiC 出现的进化途径及其传播途径。
基于广东（GD）416 株临床分离的铜绿假单胞菌新测序株、282 株中国分离株和 868 株来自其他国家的 HiRiC 分离株的基因组序列，进行系统发育分析。对全球 HiRiC ST244 进行基因组比较研究，以检测与区域特异性抗生素耐药基因相关的全球传播和局部分离模型。此外，利用最近共同祖先（MRCA）分析方法，探讨新兴的、中国特有的 HiRiC ST1971 的进化途径。
基于比较基因组学分析，我们发现 ST244 分离株存在明显的地理分离，但广东和美洲的 ST244 分离株存在关联。我们确定了一组 38 个 AMR 基因，这些基因与 ST244 的地理分离有关，并且我们确定了与 GD ST244 呈正相关（MexB）和负相关（opmD）的遗传决定因素。对于中国特有的 HiRiC ST1971，我们还推断了其在成为中国 ST1971 之前在不同大陆的进化历史。
本研究深入了解了全球传播的铜绿假单胞菌 HiRiC（ST244）之间存在区域差异的具体遗传学基础，以及区域性 HiRiC（ST1971）传播和进化的新认识。了解这些和其他 HiRiC 的遗传学可能有助于控制其出现和进一步传播。
