Department of Surgery, Kaohsiung Veterans General Hospital, Kaohsiung 813, Taiwan; Institute of Biomedical Sciences, National Sun Yat-sen University, Kaohsiung 804, Taiwan.
Department of Surgery, Kaohsiung Municipal Minsheng Hospital, Kaohsiung, Taiwan.
Biochem Pharmacol. 2023 Nov;217:115803. doi: 10.1016/j.bcp.2023.115803. Epub 2023 Sep 13.
Breast cancer stands as the most prevalent and heterogeneous malignancy affecting women globally, posing a substantial health concern. Enhanced comprehension of tumor pathology and the development of novel therapeutics are pivotal for advancing breast cancer treatment. Contemporary breast cancer investigation heavily leans on in vivo models and conventional cell culture techniques. Nonetheless, these approaches often encounter high failure rates in clinical trials due to species disparities and tissue structure variations. To address this, three-dimensional cultivation of organoids, resembling organ-like structures, has emerged as a promising alternative. Organoids represent innovative in vitro models that mirror in vivo tissue microenvironments. They retain the original tumor's diversity and facilitate the expansion of tumor samples from diverse origins, facilitating the representation of varying tumor stages. Optimized breast cancer organoid models, under precise culture conditions, offer benefits including convenient sample acquisition, abbreviated cultivation durations, and genetic stability. These attributes ensure a faithful replication of in vivo traits of breast cancer cells. As intricate cellular entities boasting spatial arrangements, breast cancer organoid models harbor substantial potential in precision medicine, organ transplantation, modeling intricate diseases, gene therapy, and drug innovation. This review delivers an overview of organoid culture techniques and outlines future prospects for organoid modeling.
乳腺癌是全球女性最常见且异质性最强的恶性肿瘤，对健康构成重大威胁。深入了解肿瘤病理学和开发新的治疗方法对于推进乳腺癌治疗至关重要。目前的乳腺癌研究主要依赖于体内模型和传统的细胞培养技术。然而，由于物种差异和组织结构的变化，这些方法在临床试验中经常失败。为了解决这个问题，类器官的三维培养已经成为一种很有前途的替代方法。类器官是类似于器官结构的创新体外模型，它们可以模拟体内组织的微环境。它们保留了原始肿瘤的多样性，并促进了来自不同来源的肿瘤样本的扩增，从而可以更好地代表不同的肿瘤阶段。在精确的培养条件下，优化的乳腺癌类器官模型具有方便的样本获取、缩短的培养时间和遗传稳定性等优点。这些特性确保了对乳腺癌细胞体内特性的忠实复制。作为具有空间排列的复杂细胞实体，乳腺癌类器官模型在精准医学、器官移植、模拟复杂疾病、基因治疗和药物创新方面具有巨大的潜力。本综述介绍了类器官培养技术，并概述了类器官建模的未来前景。
