"Luiz de Queiroz" College of Agriculture, University of São Paulo, São Paulo, Brazil.
Rice Research Station, Louisiana State University AgCenter, Baton Rouge, USA.
Sci Rep. 2023 Sep 14;13(1):15216. doi: 10.1038/s41598-023-41728-8.
Raspberries (Rubus spp) are temperate climate fruits with profitable high returns and have the potential for diversification of fruit growing in mid to low-latitude regions. However, there are still no cultivars adapted to climatic conditions and high pressure of diseases that occurs in tropical areas. In this context, our objective was to evaluate the genetic diversity from a 116 raspberry genotypes panel obtained from interspecific crosses in a testcross scheme with four cultivars already introduced in Brazil. The panel was genotyped via genotyping-by-sequencing. 28,373 and 27,281 SNPs were obtained, using the species R. occidentalis and R. idaeus genomes as references, respectively. A third marker dataset was constructed consisting of 41,292 non-coincident markers. Overall, there were no differences in the results when using the different marker sets for the subsequent analyses. The mean heterozygosity was 0.54. The average effective population size was 174, indicating great genetic variability. The other analyses revealed that the half-sibling families were structured in three groups. It is concluded that the studied panel has great potential for breeding and further genetic studies. Moreover, only one of the three marker matrices is sufficient for diversity studies.
覆盆子（Rubus spp）是一种温带气候水果，具有高收益和利润丰厚的特点，并且有潜力在中低纬度地区实现水果种植的多样化。然而，在热带地区，仍然没有适应气候条件和高疾病压力的品种。在这种情况下，我们的目标是评估来自 116 个覆盆子基因型的panel 的遗传多样性，该 panel 是通过种间杂交获得的，并采用测试交配方案与已经引入巴西的四个品种进行杂交。该 panel 通过测序分型进行基因分型。使用物种 R. occidentalis 和 R. idaeus 基因组作为参考，分别获得了 28,373 和 27,281 个 SNPs。构建了第三个标记数据集，其中包含 41,292 个非重叠标记。总体而言，在随后的分析中使用不同的标记集时，结果没有差异。平均杂合度为 0.54。有效种群大小的平均值为 174，表明遗传多样性很大。其他分析表明，半同胞家系分为三组。因此，可以得出结论，所研究的 panel 具有很大的育种和进一步遗传研究的潜力。此外，三种标记矩阵中的任何一种都足以进行多样性研究。
