具核梭杆菌的比较基因组分析揭示了种内高度多样性及核心基因组多位点序列分型（cgmlst）标记构建。
Comparative genomic analysis of Fusobacterium nucleatum reveals high intra-species diversity and cgmlst marker construction.
作者信息
Zhu Qianhui, Dovletgeldiyev Arslan, Shen Chen, Li Kexin, Hu Songnian, He Zilong
机构信息
School of Engineering Medicine, Beihang University, No. 37 Xueyuan Road, Haidian District, Beijing, 100191, China.
Beijing Advanced Innovation Center for Big Data-Based Precision Medicine, Interdisciplinary Innovation Institute of Medicine and Engineering, Beihang University, Beijing, China.
出版信息
Gut Pathog. 2023 Sep 14;15(1):43. doi: 10.1186/s13099-023-00570-z.
BACKGROUND
Fusobacterium nucleatum is a one of the most important anaerobic opportunistic pathogens in the oral and intestinal tracts of human and animals. It can cause various diseases such as infections, Lemierre's syndrome, oral cancer and colorectal cancer. The comparative genomic studies on the population genome level, have not been reported.
RESULTS
We analyzed all publicly available Fusobacterium nucleatums' genomic data for a comparative genomic study, focusing on the pan-genomic features, virulence genes, plasmid genomes and developed cgmlst molecular markers. We found the pan-genome shows a clear open tendency and most of plasmids in Fusobacterium nucleatum are mainly transmitted intraspecifically.
CONCLUSIONS
Our comparative analysis of Fusobacterium nucleatum systematically revealed the open pan-genomic features and phylogenetic tree based on cgmlst molecular markers. What's more, we also identified common plasmid typing among genomes. We hope that our study will provide a theoretical basis for subsequent functional studies.
背景
具核梭杆菌是人和动物口腔及肠道中最重要的厌氧机会性病原体之一。它可引发多种疾病，如感染、勒米埃尔综合征、口腔癌和结直肠癌。目前尚未见关于具核梭杆菌群体基因组水平的比较基因组学研究报道。
结果
我们分析了所有公开可用的具核梭杆菌基因组数据以进行比较基因组学研究，重点关注泛基因组特征、毒力基因、质粒基因组并开发了cgmlst分子标记。我们发现泛基因组呈现明显的开放趋势，且具核梭杆菌中的大多数质粒主要在种内传播。
结论
我们对具核梭杆菌的比较分析系统地揭示了基于cgmlst分子标记的开放泛基因组特征和系统发育树。此外，我们还鉴定了基因组间常见的质粒分型。我们希望我们的研究能为后续的功能研究提供理论依据。
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