Hoang Chi K, Le Cuong H, Nguyen Dat T, Tran Hang T N, Luu Chinh V, Le Huong M, Tran Ha T H
Institute of Natural Products Chemistry, Vietnam Academy of Science and Technology, Hanoi, Vietnam.
Center for Research and Technology Transfer, Vietnam Academy of Science and Technology, Hanoi, Vietnam.
Mycobiology. 2023 Aug 31;51(4):246-255. doi: 10.1080/12298093.2023.2248717. eCollection 2023.
Genus comprising the most important and extensively studied fungi has been well-known as a rich source of secondary metabolites. Our study aimed to analyze and investigate biological activities, including anti-cancer, anti-inflammatory and anti-diabetic properties, of metabolites from a marine-derived fungus belonging to . The chemical compounds in the culture broth of strain N33.2 were extracted with ethyl acetate. Followingly, chemical analysis of the extract leaded to the isolation of three ergostane-type steroid components, namely cerevisterol , ergosterol peroxide , and (3β,5α,22E)-ergosta-6,8(14),22-triene-3,5-diol . Among these, was the most potent cytotoxic against human cancer cell lines Hep-G2, A549 and MCF-7 with IC values of 2.89, 18.51, and 16.47 µg/mL, respectively, while the compound showed no significant effect against tested cancer cells. Anti-inflammatory properties of purified compounds were evaluated based on NO-production in LPS-induced murine RAW264.7 macrophages. As a result, tested compounds performed diverse inhibitory effects on NO production by the macrophages, with the most significant inhibition rate of 81.37 ± 1.35% at 25 µg/mL by the compound . Interestingly, compounds and exhibited inhibitory activities against pancreatic lipase and α-glucosidase enzymes assays. Our study brought out new data concerning the chemical properties and biological activities of isolated steroids from a fungus.
属包含最重要且被广泛研究的真菌，一直作为次生代谢产物的丰富来源而闻名。我们的研究旨在分析和研究一种源自海洋的属于该属的真菌代谢产物的生物活性，包括抗癌、抗炎和抗糖尿病特性。菌株N33.2培养液中的化合物用乙酸乙酯萃取。随后，对提取物进行化学分析，分离出三种麦角甾烷型甾体成分，即酵母甾醇、过氧化麦角甾醇和(3β,5α,22E)-麦角甾-6,8(14),22-三烯-3,5-二醇。其中，对人癌细胞系Hep-G2、A549和MCF-7的细胞毒性最强，IC值分别为2.89、18.51和16.47µg/mL，而该化合物对测试的癌细胞无显著影响。基于脂多糖诱导的小鼠RAW264.7巨噬细胞中一氧化氮的产生来评估纯化化合物的抗炎特性。结果，测试化合物对巨噬细胞产生一氧化氮表现出不同的抑制作用，化合物在25µg/mL时的最大抑制率为81.37±1.35%。有趣的是，化合物和在胰脂肪酶和α-葡萄糖苷酶活性测定中表现出抑制活性。我们的研究给出了关于从该属真菌中分离出的甾体的化学性质和生物活性的新数据。
