College of Chemistry, Chemical Engineering and Materials Science, Key Laboratory of Molecular and Nano Probes, Ministry of Education, Shandong Normal University, Jinan 250014, PR China.
College of Chemistry, Chemical Engineering and Materials Science, Key Laboratory of Molecular and Nano Probes, Ministry of Education, Shandong Normal University, Jinan 250014, PR China.
Food Chem. 2024 Feb 15;434:137461. doi: 10.1016/j.foodchem.2023.137461. Epub 2023 Sep 11.
Herein, we report a novel differential ratiometric molecularly imprinted polymer (MIP) electrochemical sensor for sulfadiazine (SDZ). An MIP membrane with double templates, SDZ and propyl gallate (PG), was fabricated on glassy carbon electrode (GCE) modified by CuInS/ZnS nanocomposites. After adding PG in the samples as the reference, the current differences between MIP@CuInS/ZnS/GCE and non-imprinted polymer@CuInS/ZnS/GCE at the potentials of 0.18 V (ΔI) and 0.92 V (ΔI) were measured. The ratio of ΔI/ΔI was used for SDZ determination in the differential and ratiometric dual-mode. The influence of the variations in electrode modification and sample enrichment conditions on the determination of SDZ can be suppressed by 2.8 ∼ 13.2-fold, enhancing the reproducibility and stability of the MIP sensor. The interference level was reduced by one order of magnitude compared with the normal MIP mode. The proposed sensors were used to determine SDZ in food samples, with the detection limit of 2.1 nM.
在此，我们报告了一种用于磺胺嘧啶（SDZ）的新型差分比率分子印迹聚合物（MIP）电化学传感器。在玻璃碳电极（GCE）上修饰的 CuInS/ZnS 纳米复合材料上制备了具有双模板（SDZ 和没食子酸丙酯（PG））的 MIP 膜。在样品中加入 PG 作为参比后，在 0.18 V（ΔI）和 0.92 V（ΔI）的电位下测量 MIP@CuInS/ZnS/GCE 和非印迹聚合物@CuInS/ZnS/GCE 之间的电流差（ΔI）。通过差分和比率双模，将ΔI/ΔI 的比值用于 SDZ 的测定。通过 2.8∼13.2 倍的比率，可以抑制电极修饰和样品富集条件变化对 SDZ 测定的影响，从而提高 MIP 传感器的重现性和稳定性。与正常 MIP 模式相比，干扰水平降低了一个数量级。所提出的传感器用于测定食品样品中的 SDZ，检测限为 2.1 nM。
