敲低 LIMD2 通过 ERK1/2 通路抑制卵巢癌细胞的进展。

Knockdown of LIMD2 inhibits the progression of ovarian carcinoma through ERK1/2 pathway.

机构信息

Department of Gynecology, Affiliated Hospital of Jining Medical University, 89 Guhuai Road, Jining, Shandong, 272029, People's Republic of China.

Department of Clinical Medicine, Jining Medical University, Jining, Shandong, 272067, People's Republic of China.

出版信息

Mol Biol Rep. 2023 Nov;50(11):8985-8993. doi: 10.1007/s11033-023-08733-6. Epub 2023 Sep 16.

DOI:10.1007/s11033-023-08733-6

PMID:37716918

Abstract

BACKGROUND

The incidence rate of ovarian carcinoma (OC) is the third of the female reproductive system malignant tumors, while its mortality rate ranks first among causes of female reproductive system tumor related death in the world.

METHODS

In the present research, we investigated the specific role of LIMD2 through LIMD2 knockdown in OC cells.

RESULTS

The results of online analysis and expression detection proved that LIMD2 was up-regulated in human OC tissues and cells. Knockdown of LIMD2 inhibited the proliferation, migration and invasion in OC cells. LIMD2 knockdown promoted the apoptosis, as well as the expression of Cleaved-Caspase3 and Bax. Importantly, knockdown of LIMD2 promotes cell autophagy. LC3-II/I ratio and Beclin1 expression increased in LIMD2 knockdown cells, while P62 expression declined in LIMD2 knockdown cells. Additionally, the phosphorylation of ERK1/2 was inhibited by the knockdown of LIMD2 in SKOV3 and OVCAR3 cells.

CONCLUSION

Knockdown of LIMD2 inhibits cell proliferation, migration, invasion and autophagy, and promotes the apoptosis through the ERK1/2 signaling pathway, suggesting that LIMD2-siRNA may be an effective molecule to prevent OC progression.

摘要

背景

卵巢癌（OC）的发病率在女性生殖系统恶性肿瘤中排名第三，但其死亡率在全球女性生殖系统肿瘤相关死亡原因中排名第一。

方法

在本研究中，我们通过在 OC 细胞中敲低 LIMD2 来研究 LIMD2 的具体作用。

结果

在线分析和表达检测的结果证明，LIMD2 在人 OC 组织和细胞中上调。敲低 LIMD2 抑制 OC 细胞的增殖、迁移和侵袭。LIMD2 敲低促进细胞凋亡，以及 Cleaved-Caspase3 和 Bax 的表达。重要的是，敲低 LIMD2 促进细胞自噬。LC3-II/I 比值和 Beclin1 表达在 LIMD2 敲低细胞中增加，而 P62 表达在 LIMD2 敲低细胞中下降。此外，LIMD2 在 SKOV3 和 OVCAR3 细胞中的敲低抑制了 ERK1/2 的磷酸化。