Zhu Houwei, Qu Yanqing
Department of Thyroid Surgery, Yantaishan Hospital, Yantai, Shandong 264000, P.R. China.
Oncol Lett. 2020 Feb;19(2):1241-1246. doi: 10.3892/ol.2019.11223. Epub 2019 Dec 17.
Expression levels of autophagy-related genes ARHI and Beclin1 in thyroid cancer and their relationship with clinical pathology and prognosis were investigated. The expression levels of ARHI and Beclin1 proteins in 80 cases of thyroid cancer and adjacent tissues were detected by western blot analysis. According to the expression levels of ARHI and Beclin1, low- and high-expression groups were determined and the relationship of the expression levels with the pathological parameters and prognosis in thyroid cancer was compared between the two groups. The correlation between the ARHI and Beclin1 protein expression level was analyzed by Pearsons correlation analysis. The levels of ARHI and Beclin1 proteins in thyroid cancer tissues were significantly lower than those in adjacent tissues (P<0.05). There was a significant difference in the expression levels of ARHI and Beclin1 in terms of pathological stage and differentiation degree of cancer tissues (P<0.001); however, there was no significant difference in the expression levels of ARHI and Beclin1 for different types of cancer tissues (P>0 05). There was a positive correlation between the expression levels of Beclin1 and ARHI (r=0.5187, P<0.001). The 3-year survival rates of patients with low-expression level of ARHI and Beclin1 proteins were significantly lower than those of patients with high expression (P<0.05). In conclusion, the expression levels of Beclin1 and ARHI were low in thyroid cancer, and were significantly associated with the pathological stage, differentiation degree and prognosis in thyroid cancer. Beclin1 and ARHI can be used as predictors for the development and prognosis of thyroid cancer.
研究甲状腺癌中自噬相关基因ARHI和Beclin1的表达水平及其与临床病理和预后的关系。采用蛋白质免疫印迹法检测80例甲状腺癌及癌旁组织中ARHI和Beclin1蛋白的表达水平。根据ARHI和Beclin1的表达水平确定低表达组和高表达组，并比较两组中表达水平与甲状腺癌病理参数及预后的关系。采用Pearson相关分析ARHI与Beclin1蛋白表达水平之间的相关性。甲状腺癌组织中ARHI和Beclin1蛋白水平明显低于癌旁组织（P<0.05）。ARHI和Beclin1在癌组织的病理分期和分化程度方面的表达水平有显著差异（P<0.001）；然而，不同类型癌组织中ARHI和Beclin1的表达水平无显著差异（P>0.05）。Beclin1与ARHI的表达水平呈正相关（r=0.5187，P<0.001）。ARHI和Beclin1蛋白低表达水平患者的3年生存率明显低于高表达患者（P<0.05）。综上所述，甲状腺癌中Beclin1和ARHI表达水平较低，且与甲状腺癌的病理分期、分化程度及预后显著相关。Beclin1和ARHI可作为甲状腺癌发生发展及预后的预测指标。
