Cui Long, Wang Xiaochuan, Zhao Xin, Kong Chenchen, Li Zhengchen, Liu Yangsui, Jiang Xinchun, Zhang Xinhui
Department of Hepatopancreatobiliary Surgery, Xuzhou Central Hospital Affiliated to Xuzhou Medical University Xuzhou, Jiangsu Province, China.
Int J Clin Exp Pathol. 2019 Aug 1;12(8):2989-2996. eCollection 2019.
To investigate the role of the autophagy-related genes Beclin1 and LC3 in the prognosis of pancreatic cancer.
A total of 86 pancreatic cancer tissues and 84 paired, adjacent normal pancreatic tissues were collected from 86 patients who underwent pancreatic resection surgery in our hospital from January 2009 to August 2011. Demographic data including age, gender, family cancer history, and clinic-pathological characteristics, including tumor diameter, differential, TNM staging and lymphatic metastasis were collected. The expressions of Beclin1 and LC3 were determined using both immunohistochemistry (IHC) and RT-qPCR.
The expression levels of both Beclin1 and LC3 mRNA and proteins were significantly up-regulated in the tumor tissues compared with the normal tissues. Higher expressions of Beclin1 and LC3 were found in the tumor tissues of patients with TNM stages III~IV, patients with lymphatic metastasis, and patients who died. Meanwhile Beclin1 and LC3 correlated with TNM stage, differential condition, and the patients' lymphatic metastasis rates. A survival analysis showed that patients with low expressions of Beclin1 and LC3 had longer survival times, and both the Beclin1 and LC3 genes were independent risk factors for 5-year mortality in pancreatic cancer patients.
The Beclin1 and LC3 genes correlate with the tumor stage, metastasis conditions, and pancreatic cancer patients' mortality.
探讨自噬相关基因Beclin1和LC3在胰腺癌预后中的作用。
收集2009年1月至2011年8月在我院接受胰腺切除术的86例患者的86份胰腺癌组织及84份配对的相邻正常胰腺组织。收集包括年龄、性别、家族癌症史等人口统计学数据以及包括肿瘤直径、分化程度、TNM分期和淋巴转移等临床病理特征。采用免疫组织化学（IHC）和RT-qPCR检测Beclin1和LC3的表达。
与正常组织相比，肿瘤组织中Beclin1和LC3的mRNA及蛋白表达水平均显著上调。在TNM分期为III~IV期的患者、有淋巴转移的患者以及死亡患者的肿瘤组织中发现Beclin1和LC3的表达较高。同时，Beclin1和LC3与TNM分期、分化情况以及患者的淋巴转移率相关。生存分析显示，Beclin1和LC3低表达的患者生存时间更长，且Beclin1和LC3基因均为胰腺癌患者5年死亡率的独立危险因素。
Beclin1和LC3基因与肿瘤分期、转移情况及胰腺癌患者的死亡率相关。
