Pasechnik V A
J Chromatogr. 1986 Sep 12;364:359-68. doi: 10.1016/s0021-9673(00)96226-5.
A chromatographic method has been developed for obtaining interferon products of three types: with a specific activity of 10(5) I.U./mg of protein (on the basis of leukocyte interferon); with a specific activity of 10(6) I.U./mg of protein (on the basis of leukocyte interferon); a near-homogeneous preparation with an activity of 2 X 10(8)-4 X 10(8) I.U./mg of protein (for leukocyte and recombinant interferons). In the first two cases, only gel permeation chromatography (GPC) is used, which yields interferon preparations containing the oligomeric forms of interferon and also such biologically active proteins as factor D and interleukin-1. In the last case, GPC and immunoaffinity chromatography may be conveniently supplemented by interferon purification by ion exchange to remove immunoglobulins and the products formed by their hydrolytic or proteolytic decomposition.
比活性为10(5)国际单位/毫克蛋白质（基于白细胞干扰素）；比活性为10(6)国际单位/毫克蛋白质（基于白细胞干扰素）；一种近乎均质的制剂，活性为2×10(8)-4×10(8)国际单位/毫克蛋白质（针对白细胞干扰素和重组干扰素）。在前两种情况下，仅使用凝胶渗透色谱法（GPC），该方法得到的干扰素制剂含有干扰素的寡聚形式以及诸如因子D和白细胞介素-1等生物活性蛋白。在最后一种情况下，凝胶渗透色谱法和免疫亲和色谱法可方便地通过离子交换进行干扰素纯化来补充，以去除免疫球蛋白及其水解或蛋白水解分解形成的产物。
