Kruzel M L, Georgiades J A
Novaferon Labs. Inc., Stafford, Texas.
J Biol Regul Homeost Agents. 1989 Jan-Mar;3(1):20-4.
A method for the purification of natural human interferon alpha (HuIFN Alpha) is described. It involves adsorption of interferon on silicic acid and its elution with hydrophobic electrolyte solution. Thereafter, elimination of possible viruses is achieved using nonionic detergent and ultrafiltration. Interferon recovered in the ultrafiltrate is further purified on Sephacryl S-200. Fractions corresponding to molecular weights ranging from approximately 10,000 to approximately 40,000 daltons are collected and directly applied on immobilized zinc affinity gel. IFN Alpha which is eluted with the non adsorbed fraction is subjected to buffer exchange, concentration, and sterilization. The resulting solution shows high specific activity (1 x 10(7) IU) with an apparently natural composition of interferon isospecies. The uniform buffer employed through the entire purification process makes it simple, fast and reproducible.
描述了一种纯化天然人α干扰素（HuIFN Alpha）的方法。该方法包括将干扰素吸附在硅酸上，并用疏水电解质溶液洗脱。此后，使用非离子洗涤剂和超滤去除可能存在的病毒。超滤液中回收的干扰素在Sephacryl S - 200上进一步纯化。收集分子量约为10,000至约40,000道尔顿的级分，并直接应用于固定化锌亲和凝胶。用未吸附级分洗脱的α干扰素进行缓冲液交换、浓缩和灭菌。所得溶液显示出高比活性（1×10⁷IU），且干扰素异构体组成明显天然。在整个纯化过程中使用统一的缓冲液，使其简单、快速且可重复。
