Kaplan P, Abreu S L
J Interferon Res. 1985 Summer;5(3):415-22. doi: 10.1089/jir.1985.5.415.
Rat fibroblast interferon (RfIFN), produced in serum-free media, has been purified 2,000-fold to 88% purity by a simple method that involves only two chromatographic steps: controlled pore glass and phenyl agarose chromatography. Over 70% of the initial activity was recovered. Gel electrophoresis (SDS-PAGE) and silver staining of the purified RfIFN revealed two major bands of RfIFN activity corresponding to Mr of 26,000 and 22,500 daltons, respectively. A very small amount of RfIFN was also recovered from a region of the gel corresponding to a Mr of 18,500. The average specific activity of the purified material was 3.7 X 10(8) U/mg. From these results it can be calculated that the specific activity of homogenous RfIFN is 4.0 X 10(8) U/mg.
在无血清培养基中产生的大鼠成纤维细胞干扰素（RfIFN），通过一种仅涉及两个色谱步骤（可控孔径玻璃色谱和苯基琼脂糖色谱）的简单方法，已被纯化了2000倍，纯度达到88%。回收了超过70%的初始活性。纯化后的RfIFN的凝胶电泳（SDS-PAGE）和银染色显示出两条主要的RfIFN活性带，分别对应于26,000和22,500道尔顿的分子量。在凝胶中对应于18,500分子量的区域也回收了极少量的RfIFN。纯化物质的平均比活性为3.7×10⁸ U/mg。从这些结果可以计算出，均一的RfIFN的比活性为4.0×10⁸ U/mg。
