Hajiyeva Aysel, Mamedov Chingiz, Gasimov Eldar, Rzayev Fuad, Khalilov Rovshan, Ahmadian Elham, Eftehari Aziz, Cho William C
Department of Biophysics and Biochemistry, Baku State University, Baku, Azerbaijan.
Department of Zoology and Physiology, Baku State University, Baku, Azerbaijan.
Ecotoxicol Environ Saf. 2023 Oct 1;264:115477. doi: 10.1016/j.ecoenv.2023.115477. Epub 2023 Sep 15.
During the development of nanotechnology, the production of many substances containing nanoparticles leads to the release of various nanoparticles into the environment, including the water ecosystem. The main goal of the current research was to study the ultrastructural characteristics of the entry and bioaccumulation of FeO nanoparticles in the small intestine of Cyprinus carpio (Linnaeus, 1758), as well as the pathomorphological changes in the fish organism. Two different doses (10 and 100 mg) of FeO nanoparticles were fed to fingerlings for 7 days and then intestinal samples were taken and studied. It was found that the extent of damages was boosted within the increment of nanoparticle concentration. The sequence and bioaccumulation of FeO nanoparticles in the small intestine of fish occurred as below: firstly, the nanoparticles passed into microvilli located in the apical part of enterocytes in the mucosa layer, from there into the cytoplasm of the epithelial cells, including cytoplasmatic organelles (nucleus, mitochondria, lysosomes, fat granules), and then into a lamina propria of the mucosa of the small intestine and passed into the endothelium of the blood vessels and to the erythrocytes of the vessels which located in the lumen. It was determined that although the nanoparticles were up to 30 nm in size, only particles with a maximum size of 20 nm could penetrate the intestinal wall. Thus, the release of FeO nanoparticles into the environment in high doses has a negative effect on the living ecosystem, including the body of fish living in the water.
在纳米技术发展过程中，许多含纳米颗粒物质的生产导致各种纳米颗粒释放到环境中，包括水生态系统。当前研究的主要目标是研究FeO纳米颗粒在鲤（Linnaeus，1758）小肠中的进入及生物累积的超微结构特征，以及鱼体的病理形态学变化。将两种不同剂量（10和100毫克）的FeO纳米颗粒投喂给幼鱼7天，然后采集肠道样本并进行研究。结果发现，损伤程度随着纳米颗粒浓度的增加而加剧。FeO纳米颗粒在鱼小肠中的进入及生物累积过程如下：首先，纳米颗粒进入位于黏膜层肠上皮细胞顶端的微绒毛，从那里进入上皮细胞的细胞质，包括细胞质细胞器（细胞核、线粒体、溶酶体、脂肪颗粒），然后进入小肠黏膜的固有层，再进入血管内皮并到达位于管腔中的血管红细胞。已确定，尽管纳米颗粒尺寸达30纳米，但只有最大尺寸为20纳米的颗粒才能穿透肠壁。因此，高剂量将FeO纳米颗粒释放到环境中会对包括生活在水中的鱼体在内的生物生态系统产生负面影响。
