Mansouri Borhan, Maleki Afshin, Davari Behroz, Johari Seyed Ali, Shahmoradi Behzad, Mohammadi Ebrahim, Shahsavari Siros
Student Research Committee, Kurdistan University of Medical Sciences, Sanandaj, Iran.
Environmental Health Research Center, Kurdistan University of Medical Sciences, Sanandaj, Iran.
Environ Monit Assess. 2016 Oct;188(10):575. doi: 10.1007/s10661-016-5579-6. Epub 2016 Sep 20.
The aim of this research was to investigate the coexposure of nanoparticles of titanium dioxide (TiO2) and copper oxide (CuO) on the alterations of the gill, intestine, kidney, and liver tissues of carps (Cyprinus carpio). In this study, carps (length 23 ± 1.5 cm; weight 13 ± 1.3 g) were divided into six groups of 15 each and exposed to 2.5 and 5.0 mg L(-1) of CuO nanoparticles (NPs), 10.0 mg L(-1) of TiO2 NPs, and 2.5 and 5.0 mg L(-1) of CuO NPs + 10.0 mg L(-1) of TiO2 NP mixture. Fish were sampled for histopathological studies after hematoxylin-eosin staining. Results indicated that the more kinds of histopathology anomalies observed with CuO NP and TiO2 NP mixture were broadly of the same type as CuO NPs and TiO2 NPs alone, but the severity or incidence of injuries of gill, intestine, liver, and kidney of carps in the mixture of CuO NPs + TiO2 NPs was higher than that of each NP alone. Moreover, behavioral changes in carps exposed to CuO NP and TiO2 NP mixture such as hyperactivity, loss of balance, and convulsions were higher than those to CuO NPs and TiO2 NPs alone. In conclusion, the presence of TiO2 NPs enhanced the effects of NPs of copper oxide in terms of histopathological changes in carps.
本研究旨在探究二氧化钛（TiO₂）纳米颗粒与氧化铜（CuO）纳米颗粒共同暴露对鲤鱼（Cyprinus carpio）鳃、肠道、肾脏和肝脏组织变化的影响。在本研究中，将鲤鱼（体长23 ± 1.5厘米；体重13 ± 1.3克）分为六组，每组15条，分别暴露于2.5毫克/升和5.0毫克/升的CuO纳米颗粒（NPs）、10.0毫克/升的TiO₂ NPs，以及2.5毫克/升和5.0毫克/升的CuO NPs + 10.0毫克/升的TiO₂ NP混合物中。经苏木精-伊红染色后对鱼进行组织病理学研究取样。结果表明，CuO NP和TiO₂ NP混合物观察到的组织病理学异常种类与单独的CuO NPs和TiO₂ NPs大致相同，但CuO NPs + TiO₂ NPs混合物中鲤鱼鳃、肠道、肝脏和肾脏的损伤严重程度或发生率高于单独的每种NP。此外，暴露于CuO NP和TiO₂ NP混合物的鲤鱼的行为变化，如多动、失去平衡和抽搐，比单独暴露于CuO NPs和TiO₂ NPs的鲤鱼更高。总之，就鲤鱼的组织病理学变化而言，TiO₂ NPs的存在增强了氧化铜纳米颗粒的影响。
