Faustova Mariia, Nazarchuk Oleksandr, Dmytriiev Dmytro, Babina Yuliana, Nazarchuk Halyna, Dudar Alina
Department of Microbiology, Virology and Immunology, Poltava State Medical University, Poltava, Ukraine.
Department of Microbiology, National Pirogov Memorial Medical University, Vinnytsia, Ukraine.
Front Microbiol. 2023 Aug 29;14:1199899. doi: 10.3389/fmicb.2023.1199899. eCollection 2023.
Today surgical site infections (SSIs) remain the second among hospital acquired infections in Europe and the USA. as a pathogen of nosocomial infections occur more frequently in surgical hospitals. The work was aimed to establish the effect of local anesthetics against planktonic forms and biofilm-formation of clinical strains and the relationship between the sensitivity of strains to local anesthetics and antiseptics . The antimicrobial activity of local anesthetics (0.5%, bupivacaine, 2.0% lidocaine, 0.375% ropivacaine) and antiseptics (decamethoxine 0.02%, chlorhexidine 0.05%) against clinical strains of was observed and studied their ability to produce biofilms. The antimicrobial effect of local anesthetics was lower compared to antiseptics, but we observed inhibition of growth and reproduction of in their presence. The ropivacaine solution and the lidocaine solution demonstrated almost the same activity against the studied microorganism isolates. Along with this, bupivacaine solution had the highest activity against the studied microorganisms. The minimal inhibitory concentration of bupivacaine for was 2.2 times lower than the minimal inhibitory concentration of lidocaine and 2.1 times lower than the minimal inhibitory concentration of ropivacaine significantly ( < 0.05). Scientific research on various aspects of the formation of bacterial biofilms is a relevant area that will change approaches to the prophylaxis and treatment of a number of infections, including SSIs.
如今，手术部位感染（SSIs）在欧洲和美国仍是医院获得性感染中的第二大类型。作为医院感染的病原体，其在外科医院中更为频繁地出现。这项工作旨在确定局部麻醉剂对临床菌株浮游形式和生物膜形成的影响，以及菌株对局部麻醉剂和防腐剂敏感性之间的关系。观察了局部麻醉剂（0.5%布比卡因、2.0%利多卡因、0.375%罗哌卡因）和防腐剂（0.02%十甲溴铵、0.05%氯己定）对临床菌株的抗菌活性，并研究了它们产生生物膜的能力。与防腐剂相比，局部麻醉剂的抗菌效果较低，但我们观察到在其存在的情况下会抑制其生长和繁殖。罗哌卡因溶液和利多卡因溶液对所研究的微生物分离株表现出几乎相同的活性。与此同时，布比卡因溶液对所研究的微生物具有最高的活性。布比卡因对的最低抑菌浓度比利多卡因的最低抑菌浓度低2.2倍，比罗哌卡因的最低抑菌浓度低2.1倍，差异具有统计学意义（<0.05）。关于细菌生物膜形成各个方面的科学研究是一个相关领域，它将改变包括SSIs在内的许多感染的预防和治疗方法。
