母亲母乳喂养与后代微生物群多样性相关；MicrobeMom随机对照试验的二次分析
Maternal breastfeeding is associated with offspring microbiome diversity; a secondary analysis of the MicrobeMom randomized control trial.
作者信息
Yelverton Cara A, Killeen Sarah Louise, Feehily Conor, Moore Rebecca L, Callaghan Shauna L, Geraghty Aisling A, Byrne David F, Walsh Calum J, Lawton Elaine M, Murphy Eileen F, Van Sinderen Douwe, Cotter Paul D, McAuliffe Fionnuala M
机构信息
UCD Perinatal Research Centre, School of Medicine, University College Dublin, National Maternity Hospital, Dublin, Ireland.
APC Microbiome Ireland, National University of Ireland, Cork, Ireland.
出版信息
Front Microbiol. 2023 Aug 31;14:1154114. doi: 10.3389/fmicb.2023.1154114. eCollection 2023.
BACKGROUND
Microbial dysbiosis in infancy can influence long-term health outcomes such as childhood obesity. The aim of this study is to explore relationships among maternal well-being during pregnancy, breastfeeding, and the infant gut microbiome.
METHODS
This is a secondary analysis of healthy pregnant women from the MicrobeMom study, a double-blind randomized control trial of maternal probiotic supplementation ( 702258) versus placebo antenatally and up to 3 months postpartum. Maternal well-being was assessed using the WHO-5 well-being index at 16 weeks' and 34 weeks' gestation. Breastfeeding practices were recorded at discharge from hospital and at 1 month postpartum. Infant stool samples were obtained at 1 month of age. Next generation shotgun sequencing determined infant microbial diversity. Independent sample -tests and Mann-Whitney tests informed adjusted regression analysis, which was adjusted for delivery mode, antibiotics during delivery, maternal age and body mass index (BMI), and probiotic vs. control study group.
RESULTS
Women ( = 118) with at least one measure of well-being were on average 33 years (SD 3.93) of age and 25.09 kg/m (SD 3.28) BMI. Exclusive breastfeeding was initiated by 65% ( = 74). Any breastfeeding was continued by 69% ( = 81) after 1 month. In early and late pregnancy, 87% ( = 97/111) and 94% ( = 107/114) had high well-being scores. Well-being was not associated with infant microbial diversity at 1 month. In adjusted analysis, exclusive breastfeeding at discharge from hospital was associated with infant microbial beta diversity (PC2; 0.254, 95% CI 0.006, 0.038). At 1 month postpartum, any breastfeeding was associated with infant microbial alpha diversity (Shannon index; -0.241, 95% CI -0.498, -0.060) and observed species; (-0.325, 95% CI -0.307, -0.060), and infant microbial beta diversity (PC2; 0.319, 95% CI 0.013, 0.045). Exclusive breastfeeding at 1 month postpartum was associated with infant alpha diversity (Shannon index -0.364, 95% CI -0.573, -0.194; Simpson index 0.339, 95% CI 0.027, 0.091), and infant's number of observed microbial species (-0.271, 95% CI -0.172, -0.037).
CONCLUSION
Breastfeeding practices at 1 month postpartum were associated with lower microbial diversity and observed species in infants at 1 month postpartum, which is potentially beneficial to allow greater abundance of .
CLINICAL TRIAL REGISTRATION
ISRCTN53023014.
背景
婴儿期的微生物群落失调会影响长期健康结果，如儿童肥胖。本研究的目的是探讨孕期母亲幸福感、母乳喂养与婴儿肠道微生物群之间的关系。
方法
这是对来自“微生物妈妈”研究的健康孕妇进行的二次分析，该研究是一项双盲随机对照试验，比较产前及产后3个月补充母体益生菌（702258）与安慰剂的效果。在妊娠16周和34周时，使用WHO-5幸福感指数评估母亲的幸福感。在出院时和产后1个月记录母乳喂养情况。在婴儿1月龄时采集粪便样本。采用新一代鸟枪法测序确定婴儿微生物多样性。独立样本t检验和曼-惠特尼检验为调整后的回归分析提供依据，该分析针对分娩方式、分娩期间使用的抗生素、母亲年龄和体重指数（BMI）以及益生菌与对照组研究组进行了调整。
结果
至少有一项幸福感指标的女性（n = 118）平均年龄为33岁（标准差3.93），BMI为25.09kg/m²（标准差3.28）。65%（n = 74）的女性开始进行纯母乳喂养。1个月后，69%（n = 81）的女性继续进行任何形式的母乳喂养。在妊娠早期和晚期，87%（n = 97/111）和94%（n = 107/114）的女性幸福感得分较高。幸福感与1月龄时婴儿的微生物多样性无关。在调整分析中，出院时的纯母乳喂养与婴儿微生物β多样性相关（PC2；0.254，95%CI 0.006，0.038）。在产后1个月，任何形式的母乳喂养与婴儿微生物α多样性（香农指数；-0.241，95%CI -0.498，-0.060）和观察到的物种相关（-0.325，95%CI -0.307，-0.060），以及婴儿微生物β多样性（PC2；0.319，95%CI 0.013，0.045）。产后1个月的纯母乳喂养与婴儿α多样性（香农指数-0.364，95%CI -0.573，-0.194；辛普森指数0.339，95%CI 0.027，0.091）以及婴儿观察到的微生物物种数量相关（-0.271，95%CI -0.172，-0.037）。
结论
产后1个月的母乳喂养方式与产后1个月婴儿较低的微生物多样性和观察到的物种数量相关，这可能有利于使某种物质的丰度更高。
临床试验注册
ISRCTN53023014
Zotero 插件