Baadsgaard Bruun Louise, Waskiw Hardon Tommy A, Havnsøe Krogh Anne K, Qvortup Klaus, Heegaard Steffen, Thuri Kristensen Annemarie, Henriksen Michala de Linde
Department of Veterinary Clinical Sciences, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark.
Anicura Aarhus Veterinary Hospital, Aarhus, Denmark.
Vet Ophthalmol. 2025 Mar;28(2):175-189. doi: 10.1111/vop.13148. Epub 2023 Sep 18.
The purpose of this case series was to describe the effect of autologous PRF membrane for corneal reconstruction surgery in dogs. PRF membranes made from two healthy dogs unrelated to the current case series were used for PRF histologic analyses.
Seven dogs with complicated corneal ulcerations.
A complete ophthalmic examination, hematology, and fibrinogen analysis were performed pre-surgery. A PRF clot was made from autologous blood in a serum tube after centrifugation in a horizontal Bio-PRF® Centrifuge at 700 × g for 8 min. The PRF clot was processed in a PRF-Box® into a PRF membrane. The PRF membrane was sutured to the corneal ulcer bed. Each dog had a follow-up at days 5-7, 12-14, and 30-40 post-surgery. A final long-term follow-up was performed as well.
A positive outcome with healing and a "good" quality PRF membrane was seen in six out of seven dogs. One dog had a fibrinogen level below normal range and the PRF membrane was of "poor" quality. This dog developed a descemetocele 13 days post-surgery and needed rescue surgery. Mean healing time for all dogs was 9 ± 5.5 days. Minimal scarring, corneal pigmentation, and vascularization were observed at the final long-term follow-up 288 ± 44 days post-surgery.
PRF membrane was successful as graft material for corneal ulceration reconstruction surgery. Low fibrinogen appeared to have negative effect on the quality of the PRF membrane, showing the importance for the surgeon to evaluate the quality of the PRF membrane prior to surgery.
本病例系列的目的是描述自体富血小板纤维蛋白（PRF）膜在犬角膜重建手术中的效果。使用来自两只与本病例系列无关的健康犬的PRF膜进行PRF组织学分析。
七只患有复杂性角膜溃疡的犬。
术前进行全面的眼科检查、血液学检查和纤维蛋白原分析。在水平式Bio-PRF®离心机中以700×g离心8分钟后，从血清管中的自体血液制成PRF凝块。将PRF凝块在PRF-Box®中加工成PRF膜。将PRF膜缝合到角膜溃疡床上。每只犬在术后第5 - 7天、12 - 14天和30 - 40天进行随访。还进行了最终的长期随访。
七只犬中有六只愈合情况良好，PRF膜质量“良好”。一只犬的纤维蛋白原水平低于正常范围，PRF膜质量“较差”。这只犬在术后13天发生了后弹力层膨出，需要进行抢救手术。所有犬的平均愈合时间为9±5.5天。在术后288±44天的最终长期随访中，观察到最小程度的瘢痕形成、角膜色素沉着和血管化。
PRF膜作为角膜溃疡重建手术的移植材料是成功的。低纤维蛋白原似乎对PRF膜的质量有负面影响，这表明外科医生在手术前评估PRF膜质量的重要性。
