Yamada K, Okamura K, Nakakuki M, Ozawa N, Yajima A
Nihon Sanka Fujinka Gakkai Zasshi. 1986 Sep;38(9):1535-9.
Antiviral activity (3-24 IU/ml) was detected in the amniotic fluid of 21 of 25 pregnant women examined (84%) by conventional interferon assay using human amniotic cells (WISH) and vesicular stomatitis virus. The pregnancies were from 16 to 42 weeks gestation without obvious viral infection or other complications. The antiviral substance in the amniotic fluid seems to be interferon alpha, because it was not inactivated by heat (56 degrees C, 30 min) or acid (pH 2.0, 24 hrs.) and it was inactivated by anti-human interferon alpha neutralizing serum. Neither the incidence nor the titer of the activity in amniotic fluid has any relationship with gestational age, age of the pregnant women or parity. Antiviral activity (4-32 IU/10 mg protein/ml) was also detected in the placenta homogenate of 23 of 25 pregnant women examined (92%). The pregnancies were from 7 to 41 weeks of gestation with or without complication. The titer of antiviral activity was relatively high in the placenta of babies with central nervous system anomalies and it was low in the placenta of intrauterine growth retardation pregnancies, compared with normal pregnancies. The activity in the placenta was not inactivated by heat (56 degrees C, 30 min), acid (pH 2.0, 24 hrs.), anti-human interferon alpha neutralizing anti-serum or anti-human interferon beta neutralizing anti-serum.
采用人羊膜细胞（WISH）和水疱性口炎病毒，通过传统干扰素检测法，在25名接受检查的孕妇中的21名（84%）的羊水内检测到抗病毒活性（3 - 24 IU/ml）。这些妊娠处于妊娠16至42周，无明显病毒感染或其他并发症。羊水内的抗病毒物质似乎是α干扰素，因为它不被热（56℃，30分钟）或酸（pH 2.0，24小时）灭活，且被抗人α干扰素中和血清灭活。羊水内活性的发生率和滴度与孕周、孕妇年龄或产次均无关系。在25名接受检查的孕妇中的23名（92%）的胎盘匀浆中也检测到抗病毒活性（4 - 32 IU/10 mg蛋白/ml）。这些妊娠处于妊娠7至41周，有或无并发症。与正常妊娠相比，中枢神经系统异常婴儿的胎盘内抗病毒活性滴度相对较高，而宫内生长受限妊娠的胎盘内抗病毒活性滴度较低。胎盘内的活性不被热（56℃，30分钟）、酸（pH 2.0，24小时）、抗人α干扰素中和抗血清或抗人β干扰素中和抗血清灭活。
