Muneoka K, Holler-Dinsmore G, Bryant S V
J Exp Zool. 1986 Oct;240(1):47-54. doi: 10.1002/jez.1402400107.
The regenerative capability of Xenopus laevis hindlimbs was studied at different developmental stages. Three types of surgical deletion of the autopod were performed: simple amputations, 3-digit wedge-shaped deletions and 1-digit wedge-shaped deletions. The frequency of regenerative response and the digital patterns of the regenerates were analyzed. Regenerative capacity declines with developmental stage for all three types of deletions. For simple amputations this decline occurs in an orderly manner with the failure of anterior digits to regenerate at progressively later stages. A comparison between 1-digit and 3-digit deletions shows that for all stages of development, 3-digit deletions regenerate better than one-digit deletions. These data indicate that the amount of tissue removed is directly related to whether a regenerative response is observed. At any given stage, larger deletions are more likely to regenerate than smaller ones. These results are discussed with regard to the mechanisms by which growth during regeneration is controlled.
研究了非洲爪蟾后肢在不同发育阶段的再生能力。对自足进行了三种类型的手术切除：简单截肢、三趾楔形切除和单趾楔形切除。分析了再生反应的频率和再生后的趾型。对于所有三种类型的切除，再生能力均随发育阶段而下降。对于简单截肢，这种下降以有序的方式发生，前趾在逐渐较晚的阶段无法再生。单趾切除和三趾切除的比较表明，在所有发育阶段，三趾切除的再生情况都比单趾切除好。这些数据表明，切除的组织量与是否观察到再生反应直接相关。在任何给定阶段，较大的切除比较小的切除更有可能再生。结合再生过程中生长的控制机制对这些结果进行了讨论。
