Post L E, Strycharz G D, Nomura M, Lewis H, Dennis P P
Proc Natl Acad Sci U S A. 1979 Apr;76(4):1697-701. doi: 10.1073/pnas.76.4.1697.
The 3072-nucleotide-long sequence of a segment from the 88-min region of the Escherichia coli chromosome has been determined. The sequence covers the genes for ribosomal proteins L11 (rplK), LI (rplA), L10 (rplJ), and L7/L12 ((rplL), and the 5' end of the gene for the beta subunit of RNA polymerase (rpoB), along with the presumed regulatory regions for these genes. The probable locations of the promoter for the first two genes (the L11 operon) and the promoter for the latter three genes (the proximal part of the beta operon) have been identified. We have also found that the four ribosomal protein genes preferentially use codons that are recognized efficiently by the most abundant tRNA species. These and other features of the sequence results are discussed in relation to available information obtained from both in vitro and in vivo experiments on the expression of these ribosomal and RNA polymerase subunit genes.
已确定大肠杆菌染色体88分钟区域一段3072个核苷酸长的序列。该序列涵盖核糖体蛋白L11(rplK)、L1(rplA)、L10(rplJ)和L7/L12(rplL)的基因,以及RNA聚合酶β亚基基因(rpoB)的5′端,还有这些基因的假定调控区域。已确定前两个基因(L11操纵子)的启动子和后三个基因(β操纵子近端部分)的启动子的可能位置。我们还发现,这四个核糖体蛋白基因优先使用最丰富的tRNA种类能有效识别的密码子。结合从这些核糖体和RNA聚合酶亚基基因表达的体外和体内实验获得的现有信息,讨论了序列结果的这些及其他特征。