• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

当大肠杆菌核糖体蛋白L10的合成量超过核糖体蛋白L7/L12时，它会迅速降解。

Escherichia coli ribosomal protein L10 is rapidly degraded when synthesized in excess of ribosomal protein L7/L12.

作者信息

Petersen C

机构信息

Institute of Microbiology, University of Copenhagen, Denmark.

出版信息

J Bacteriol. 1990 Jan;172(1):431-6. doi: 10.1128/jb.172.1.431-436.1990.

DOI:10.1128/jb.172.1.431-436.1990
PMID:2403546
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC208449/
Abstract

In Escherichia coli the genes encoding ribosomal proteins L10 and L7/12, rplJ and rplL, respectively, are cotranscribed and subject to translational coupling. Synthesis of both proteins is coordinately regulated at the translational level by binding of L10 or a complex of L10 and L7/L12 to a single target in the mRNA leader region upstream of rplJ. Unexpectedly, small deletions that inactivated the ribosome-binding site of the rplL gene carried on multicopy plasmids exerted a negative effect on expression of the upstream rplJ gene. This effect could be overcome by overproduction of L7/L12 in trans from another plasmid. This apparent stimulation resulted from stabilization of the overproduced L10 protein by L7/L12, presumably because free L10, in contrast to L10 complexed with L7/L12, is subject to rapid proteolytic decay. The contribution of this decay mechanism to the regulation of the rplJL operon is evaluated.

摘要

在大肠杆菌中，分别编码核糖体蛋白L10和L7/12的基因rplJ和rplL是共转录的，并受到翻译偶联的调控。这两种蛋白质的合成在翻译水平上通过L10或L10与L7/L12的复合物与rplJ上游mRNA前导区的单个靶标结合而协同调节。出乎意料的是，在多拷贝质粒上使rplL基因的核糖体结合位点失活的小缺失对上游rplJ基因的表达产生了负面影响。这种影响可以通过从另一个质粒反式过量表达L7/L12来克服。这种明显的刺激是由于L7/L12使过量产生的L10蛋白稳定，推测是因为与L7/L12复合的L10相比，游离的L10会迅速发生蛋白水解降解。评估了这种降解机制对rplJL操纵子调控的贡献。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/10fc/208449/8b7649fed7db/jbacter01043-0455-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/10fc/208449/67171d3afa14/jbacter01043-0455-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/10fc/208449/8b7649fed7db/jbacter01043-0455-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/10fc/208449/67171d3afa14/jbacter01043-0455-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/10fc/208449/8b7649fed7db/jbacter01043-0455-b.jpg

相似文献

1
Escherichia coli ribosomal protein L10 is rapidly degraded when synthesized in excess of ribosomal protein L7/L12.当大肠杆菌核糖体蛋白L10的合成量超过核糖体蛋白L7/L12时，它会迅速降解。
J Bacteriol. 1990 Jan;172(1):431-6. doi: 10.1128/jb.172.1.431-436.1990.
2
Long-range translational coupling in the rplJL-rpoBC operon of Escherichia coli.大肠杆菌rplJL-rpoBC操纵子中的远程翻译偶联
J Mol Biol. 1989 Mar 20;206(2):323-32. doi: 10.1016/0022-2836(89)90482-8.
3
In vivo and in vitro structural analysis of the rplJ mRNA leader of Escherichia coli. Protection by bound L10-L7/L12.大肠杆菌rplJ mRNA前导序列的体内和体外结构分析。L10-L7/L12结合的保护作用。
J Biol Chem. 1988 Oct 15;263(29):15166-75.
4
Sequences of the rplJL operon containing the L10 and L7/L12 genes from Brucella abortus.包含来自流产布鲁氏菌的L10和L7/L12基因的rplJL操纵子序列。
Gene. 1994 Mar 11;140(1):137-8. doi: 10.1016/0378-1119(94)90744-7.
5
Evidence for the ability of L10 ribosomal proteins of Salmonella typhimurium and Klebsiella pneumoniae to regulate rplJL gene expression in Escherichia coli.
FEBS Lett. 1990 Jun 4;265(1-2):129-32. doi: 10.1016/0014-5793(90)80901-t.
6
Ribosomal protein L10(L12)4 autoregulates expression of the Bacillus subtilis rplJL operon by a transcription attenuation mechanism.核糖体蛋白L10（L12）4通过转录衰减机制自动调节枯草芽孢杆菌rplJL操纵子的表达。
Nucleic Acids Res. 2015 Aug 18;43(14):7032-43. doi: 10.1093/nar/gkv628. Epub 2015 Jun 22.
7
Identification of a growth phase-dependent promoter in the rplJL operon of Streptomyces coelicolor A3(2).天蓝色链霉菌A3(2) rplJL操纵子中生长阶段依赖性启动子的鉴定
Biochim Biophys Acta. 2001 Jan 26;1517(2):243-9. doi: 10.1016/s0167-4781(00)00280-3.
8
Feedback regulation of the rplJL-rpoBC ribosomal protein operon of Escherichia coli requires a region of mRNA secondary structure.大肠杆菌rplJL - rpoBC核糖体蛋白操纵子的反馈调节需要一段mRNA二级结构区域。
J Mol Biol. 1987 Dec 5;198(3):371-81. doi: 10.1016/0022-2836(87)90287-7.
9
Deletion of C-terminal residues of Escherichia coli ribosomal protein L10 causes the loss of binding of one L7/L12 dimer: ribosomes with one L7/L12 dimer are active.删除大肠杆菌核糖体蛋白L10的C末端残基会导致一个L7/L12二聚体结合的丧失：含有一个L7/L12二聚体的核糖体具有活性。
Biochemistry. 2000 Apr 11;39(14):4075-81. doi: 10.1021/bi992621e.
10
The lethal effect of a plasmid resulting from transcriptional readthrough of rplJ from the rplKA operon in Escherichia coli.源于大肠杆菌rplKA操纵子中rplJ转录通读产生的质粒的致死效应。
Mol Gen Genet. 1983;189(2):275-81. doi: 10.1007/BF00337817.

引用本文的文献

1
In vitro reconstitution of the Escherichia coli 70S ribosome with a full set of recombinant ribosomal proteins.在体外使用一组全重组核糖体蛋白重新构成大肠杆菌 70S 核糖体。
J Biochem. 2022 Feb 21;171(2):227-237. doi: 10.1093/jb/mvab121.
2
Resuscitation of from dormancy requires hibernation promoting factor (PA4463) for ribosome preservation.从休眠状态复苏需要冬眠促进因子（PA4463）来保存核糖体。
Proc Natl Acad Sci U S A. 2017 Mar 21;114(12):3204-3209. doi: 10.1073/pnas.1700695114. Epub 2017 Mar 7.
3
Quantifying absolute protein synthesis rates reveals principles underlying allocation of cellular resources.

本文引用的文献

1
Autogenous control: ribosomal protein L10-L12 complex binds to the leader sequence of its mRNA.自体调控：核糖体蛋白L10-L12复合物与其mRNA的前导序列结合。
EMBO J. 1982;1(8):999-1004. doi: 10.1002/j.1460-2075.1982.tb01284.x.
2
Transcription of the S10 ribosomal protein operon is regulated by an attenuator in the leader.S10核糖体蛋白操纵子的转录受前导序列中一个衰减子的调控。
Cell. 1983 May;33(1):241-8. doi: 10.1016/0092-8674(83)90353-7.
3
RNA secondary structure and translation inhibition: analysis of mutants in the rplJ leader.RNA二级结构与翻译抑制：rplJ基因前导序列中突变体的分析
定量绝对蛋白质合成率揭示了细胞资源分配的基本原理。
Cell. 2014 Apr 24;157(3):624-35. doi: 10.1016/j.cell.2014.02.033.
4
Evolution of the protein stoichiometry in the L12 stalk of bacterial and organellar ribosomes.细菌和细胞器核糖体 L12 突柄中蛋白质化学计量的演变。
Nat Commun. 2013;4:1387. doi: 10.1038/ncomms2373.
5
Measurement of the rates of synthesis of three components of ribosomes of Mycobacterium fortuitum: a theoretical approach to qRT-PCR experimentation.测量耻垢分枝杆菌核糖体三个组成部分的合成速率：qRT-PCR 实验的理论方法。
PLoS One. 2010 Jul 14;5(7):e11575. doi: 10.1371/journal.pone.0011575.
6
Stability of the 'L12 stalk' in ribosomes from mesophilic and (hyper)thermophilic Archaea and Bacteria.嗜温及（超）嗜热古菌和细菌核糖体中“L12柄”的稳定性
Nucleic Acids Res. 2006;34(20):5800-14. doi: 10.1093/nar/gkl751. Epub 2006 Oct 19.
7
Specific recognition of rpsO mRNA and 16S rRNA by Escherichia coli ribosomal protein S15 relies on both mimicry and site differentiation.大肠杆菌核糖体蛋白S15对rpsO mRNA和16S rRNA的特异性识别依赖于模拟和位点区分。
Mol Microbiol. 2004 May;52(3):661-75. doi: 10.1111/j.1365-2958.2004.04005.x.
8
Concentrations of 4.5S RNA and Ffh protein in Escherichia coli: the stability of Ffh protein is dependent on the concentration of 4.5S RNA.
J Bacteriol. 1994 Dec;176(23):7148-54. doi: 10.1128/jb.176.23.7148-7154.1994.
9
Functions of the gene products of Escherichia coli.大肠杆菌基因产物的功能。
Microbiol Rev. 1993 Dec;57(4):862-952. doi: 10.1128/mr.57.4.862-952.1993.
10
Regulation by proteolysis: energy-dependent proteases and their targets.通过蛋白水解进行调控：能量依赖性蛋白酶及其作用靶点。
Microbiol Rev. 1992 Dec;56(4):592-621. doi: 10.1128/mr.56.4.592-621.1992.
EMBO J. 1984 Jul;3(7):1609-12. doi: 10.1002/j.1460-2075.1984.tb02018.x.
4
The lethal effect of a plasmid resulting from transcriptional readthrough of rplJ from the rplKA operon in Escherichia coli.源于大肠杆菌rplKA操纵子中rplJ转录通读产生的质粒的致死效应。
Mol Gen Genet. 1983;189(2):275-81. doi: 10.1007/BF00337817.
5
E. coli ribosomal protein L10 inhibits translation of L10 and L7/L12 mRNAs by acting at a single site.大肠杆菌核糖体蛋白L10通过作用于单个位点来抑制L10和L7/L12 mRNA的翻译。
Nature. 1981 Nov 12;294(5837):190-2. doi: 10.1038/294190a0.
6
Regulation of the synthesis of ribosomes and ribosomal components.核糖体及核糖体组分合成的调控。
Annu Rev Biochem. 1984;53:75-117. doi: 10.1146/annurev.bi.53.070184.000451.
7
Mutations in the rpIJ leader of Escherichia coli that abolish feedback regulation.大肠杆菌rpIJ前导序列中消除反馈调节的突变。
Cell. 1983 Feb;32(2):361-9. doi: 10.1016/0092-8674(83)90455-5.
8
Structural studies of ribosomes.核糖体的结构研究。
Prog Biophys Mol Biol. 1982;40(3):161-228. doi: 10.1016/0079-6107(82)90013-x.
9
Cleavage of structural proteins during the assembly of the head of bacteriophage T4.在噬菌体T4头部组装过程中结构蛋白的切割
Nature. 1970 Aug 15;227(5259):680-5. doi: 10.1038/227680a0.
10
The functional stability of the lacZ transcript is sensitive towards sequence alterations immediately downstream of the ribosome binding site.lacZ转录本的功能稳定性对核糖体结合位点下游紧邻区域的序列改变很敏感。
Mol Gen Genet. 1987 Aug;209(1):179-87. doi: 10.1007/BF00329856.