Office of Society Academia Collaboration for Innovation, Kyoto University, Kitashirakawa-Oiwakecho, Sakyo-ku, Kyoto, 606-8502, Japan.
Department of Food and Agriculture Science, Graduate School of Agriculture, Ryukoku University, 1-5 Yokotani, Seta Oe-cho, Otsu, Shiga 520-2194, Japan.
Int J Syst Evol Microbiol. 2023 Sep;73(9). doi: 10.1099/ijsem.0.006027.
Three strains of novel oleaginous yeast species were isolated from soil samples collected in Shiga Prefecture, Japan. The sequences of the internal transcribed spacer (ITS) region and the D1/D2 region of the large subunit (LSU) of the rRNA genes indicated that these novel yeast species are members of the genus . The results of molecular phylogenetic analysis indicated that strains 38-3 and 8s1 were closely related to . They differed by 10 nucleotide substitutions and one gap (1.77 %) in the D1/D2 region of the LSU of the rRNA genes and by 17-18 nucleotide substitutions and 10-11 gaps (5.45-5.85 %) in the ITS region. Strain 51-4 differed from the type strain of the most closely related species, , by 26 nucleotide substitutions (4.46 %) in the D1/D2 region of the LSU of the rRNA genes and by 20 nucleotide substitutions and six gaps (5.42 %) in the ITS region. The names proposed for these previously undescribed species are sp. nov. and sp. nov.
三株新型产油酵母从日本滋贺县采集的土壤样本中分离得到。内部转录间隔区（ITS）和大亚基（LSU）rRNA 基因的 D1/D2 区序列表明，这些新型酵母是 属的成员。分子系统发育分析的结果表明，菌株 38-3 和 8s1 与 关系密切。它们在 LSU 的 D1/D2 区有 10 个核苷酸取代和一个缺口（1.77%），在 ITS 区有 17-18 个核苷酸取代和 10-11 个缺口（5.45-5.85%）。菌株 51-4 与亲缘关系最密切的模式种 ，在 LSU 的 D1/D2 区有 26 个核苷酸取代（4.46%），在 ITS 区有 20 个核苷酸取代和 6 个缺口（5.42%）。这些尚未描述的新物种的命名为 sp. nov. 和 sp. nov.。
