Department of Mathematics and Science, Faculty of Agriculture and Technology, Rajamangala University of Technology Isan Surin Campus, Surin 32000, Thailand.
Department of Biological Science, Faculty of Science, Ubonratchathani University, Warin Chamrap District, Ubon Ratchathani 34190, Thailand.
Int J Syst Evol Microbiol. 2022 Aug;72(8). doi: 10.1099/ijsem.0.005488.
Seven yeast strains (UBIF12-1, UBFB13-1, SRFS56-3, SRFS57-2, SKFS62-1, SKFS66-1 and SKFS67-1) representing a single anamorphic novel yeast species were isolated from traditional Thai fermented foods in Ubon Ratchathani, Surin and Sisaket in the northeast part of Thailand. The results of analysis of the sequences of the D1/D2 region of the large subunit (LSU) rRNA gene and the internal transcribed spacer (ITS) region indicated that the seven strains showed zero to one nucleotide substitutions in the sequences of the D1/D2 region of the LSU rRNA gene, and zero to four nucleotide substitutions in the ITS region. CBS 5658 was the most closely-related species, but with 0.7-0.9% nucleotide substitutions in the D1/D2 region of the LSU rRNA gene, and 2.0-2.2% nucleotide substitution in the ITS region. The results of a phylogenetic analysis based on the concatenated ITS and D1/D2 regions confirmed that the seven strains represented a single species of the genus distinct from the other recognized species of the genus. Furthermore, the morphological, biochemical and physiological properties of the seven strains not only indicated that they represented members of the genus , but that they were separated from and the two most closely related species in the phylogenetic tree. Therefore, the seven strains were identified as representing a novel species, for which we propose the name f.a., sp. nov. The holotype is TBRC 15053 (isotype: SRFS57-2 and PYCC 9021). The MycoBank number of the novel species is 841892.
从泰国东北部乌汶府、素林府和四色菊府的传统泰式发酵食品中分离到了 7 株酵母菌株（UBIF12-1、UBFB13-1、SRFS56-3、SRFS57-2、SKFS62-1、SKFS66-1 和 SKFS67-1），代表了一个新的单倍体型酵母种。大亚基（LSU）rRNA 基因 D1/D2 区和内部转录间隔区（ITS）序列分析结果表明，这 7 株菌在 LSU rRNA 基因 D1/D2 区的序列中有零到一个核苷酸的替换，在 ITS 区中有零到四个核苷酸的替换。CBS5658 是最接近的种，但在 LSU rRNA 基因 D1/D2 区有 0.7-0.9%的核苷酸替换，在 ITS 区有 2.0-2.2%的核苷酸替换。基于 ITS 和 D1/D2 区联合序列的系统发育分析结果证实，这 7 株菌代表了一个与其他已识别种不同的属种。此外，这 7 株菌的形态学、生物化学和生理学特性不仅表明它们代表了该属的成员，而且与系统发育树中最接近的两个种 和 分离。因此，这 7 株菌被鉴定为代表一个新种，我们建议将其命名为 f.a.，sp. nov.。模式株为 TBRC 15053（等模式：SRFS57-2 和 PYCC 9021）。该新种的 MycoBank 编号为 841892。
