Thy-1抗原在大鼠乳腺细胞系体外分化中的作用。

Dulbecco R, Bologna M, Unger M

Proc Natl Acad Sci U S A. 1979 Apr;76(4):1848-52. doi: 10.1073/pnas.76.4.1848.

A line of rat mammary cells, LA7, undergoes differentiation into domes or ridges or projections under partial medium control. Immunofluorescent studies show that at the time of dome formation the LA7 cultures express the Thy-1 antigen at the borders between cells. Exposure of the cultures to certain monoclonal anti-Thy-1.1 sera prevents formation of new domes and causes disappearance of preexisting ones; antisera to whole cells produce no visible effect. Cultures exposed to anti-Thy-1 sera at effective concentrations of antibody against domes show morphological changes and ridge formation. It seems that the specific interactions of these antisera with the Thy-1 antigen redirects the differentiation program of the cells.

一株大鼠乳腺细胞系LA7，在部分培养基控制下会分化成圆顶、脊或突起。免疫荧光研究表明，在圆顶形成时，LA7培养物在细胞间边界处表达Thy-1抗原。将培养物暴露于某些抗Thy-1.1单克隆血清中可阻止新圆顶的形成，并导致已有的圆顶消失；全细胞抗血清则无明显作用。暴露于有效浓度的抗圆顶抗体的抗Thy-1血清中的培养物会出现形态变化并形成脊。这些抗血清与Thy-1抗原的特异性相互作用似乎重定向了细胞的分化程序。