Department of Biotechnology, Faculty of Science, Palacký University Olomouc, Olomouc, Czech Republic.
Institute for Genomics, Biocomputing and Biotechnology, Mississippi Agricultural and Forestry Experiment Station, Mississippi State University, Starkville, MS, USA.
J Exp Bot. 2024 Jan 1;75(1):405-421. doi: 10.1093/jxb/erad363.
The photosynthesis-induced accumulation of reactive oxygen species in chloroplasts can lead to oxidative stress, triggering changes in protein synthesis, degradation, and the assembly/disassembly of protein complexes. Using shot-gun proteomics, we identified methyl viologen-induced changes in protein abundance in wild-type Arabidopsis and oxidative stress-hypersensitive fsd1-1 and fsd1-2 knockout mutants, which are deficient in IRON SUPEROXIDE DISMUTASE 1 (FSD1). The levels of proteins that are localized in chloroplasts and the cytoplasm were modified in all lines treated with methyl viologen. Compared with the wild-type, fsd1 mutants showed significant changes in metabolic protein and chloroplast chaperone levels, together with increased ratio of cytoplasmic, peroxisomal, and mitochondrial proteins. Different responses in proteins involved in the disassembly of photosystem II-light harvesting chlorophyll a/b binding proteins were observed. Moreover, the abundance of PATELLIN 4, a phospholipid-binding protein enriched in stomatal lineage, was decreased in response to methyl viologen. Reverse genetic studies using patl4 knockout mutants and a PATELLIN 4 complemented line indicate that PATELLIN 4 affects plant responses to oxidative stress by effects on stomatal closure.
叶绿体中光合作用诱导的活性氧物质积累会导致氧化应激，引发蛋白质合成、降解以及蛋白质复合物组装/拆卸的变化。使用鸟枪法蛋白质组学，我们鉴定了在野生型拟南芥和对氧化应激敏感的 fsd1-1 和 fsd1-2 敲除突变体中，甲基紫精诱导的蛋白质丰度变化，这些突变体缺乏铁超氧化物歧化酶 1（FSD1）。用甲基紫精处理的所有系中，定位于叶绿体和细胞质的蛋白质水平发生了修饰。与野生型相比，fsd1 突变体中代谢蛋白和叶绿体伴侣蛋白的水平发生了显著变化，同时细胞质、过氧化物酶体和线粒体蛋白的比例增加。观察到与光系统 II-捕光叶绿素 a/b 结合蛋白解体相关的蛋白质的不同反应。此外，对甲基紫精的响应导致富含于气孔谱系的磷脂结合蛋白 PATELLIN 4 的丰度降低。使用 patl4 敲除突变体和 PATELLIN 4 互补系的反向遗传学研究表明，PATELLIN 4 通过对气孔关闭的影响影响植物对氧化应激的反应。
